PA1-12809

antibody from Invitrogen Antibodies

Targeting: MTA2 MTA1-L1, MTA1L1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA1-12809

Provider

Invitrogen Antibodies

Product name

MTA2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control for this product is HeLa cell lysate.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of MTA2 was achieved by transfecting HeLa with MTA2 specific siRNAs (Silencer® select Products # s17390, s17389). Western blot analysis (Fig. a) was performed using whole cell lysates from the MTA2 knockdown (siRNA treated) cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with MTA2 Polyclonal Antibody (Product # PA1-12809, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MTA2. An uncharacterized band (*) was observed at ~20kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-MTA2 Polyclonal Antibody (Product # PA1-12809) and ~75 kDa band corresponding to MTA2 was observed in all the cell lines and tissues tested along with an uncharacterized band at ~20 and 90 kDa. Whole cell lysate (40ug lysate) of Raji (Lane 1), A-431 (Lane 2), NIH: OVCAR-3 (Lane 3), IMR-32 (Lane 4), BeWo (Lane 5), HeLa (Lane 6), Mouse Ovary (Lane 7) and Rat Ovary (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The bot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).