Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- MA5-35547 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MTA2 Recombinant Rabbit Monoclonal Antibody (9A5Y7)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: GVPFSANGRP LASGIRSSSQ PAAKRQKLNP ADAPNPVVFV ATKDTRALRK ALTHLEMRRA ARRPNLPLKV KPTLIAVRPP VPLPAPSHPA STNEPIVLED
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 9A5Y7
- Vial size
- 100 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MTA2 in extracts of various cell lines. Samples were incubated with MTA2 Monoclonal antibody (Product # MA5-35547) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MTA2 in extracts from wild type (WT) and MTA2 knockout (KO) 293T cells. Samples were incubated with MTA2 Monoclonal antibody (Product # MA5-35547) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of MTA2 in U-2 OS. Samples were incubated with MTA2 Monoclonal antibody (Product # MA5-35547) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MTA2 in paraffin-embedded human colon. Samples were incubated with MTA2 Monoclonal antibody (Product # MA5-35547) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP analysis of MTA2 in extracts of HeLa cells. Samples were incubated with MTA2 Monoclonal antibody (Product # MA5-35547) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.