MA5-35547

antibody from Invitrogen Antibodies

Targeting: MTA2 MTA1-L1, MTA1L1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-35547

Provider

Invitrogen Antibodies

Product name

MTA2 Recombinant Rabbit Monoclonal Antibody (9A5Y7)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: GVPFSANGRP LASGIRSSSQ PAAKRQKLNP ADAPNPVVFV ATKDTRALRK ALTHLEMRRA ARRPNLPLKV KPTLIAVRPP VPLPAPSHPA STNEPIVLED

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

9A5Y7

Vial size

100 µL

Concentration

0.8 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MTA2 in extracts of various cell lines. Samples were incubated with MTA2 Monoclonal antibody (Product # MA5-35547) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MTA2 in extracts from wild type (WT) and MTA2 knockout (KO) 293T cells. Samples were incubated with MTA2 Monoclonal antibody (Product # MA5-35547) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of MTA2 in U-2 OS. Samples were incubated with MTA2 Monoclonal antibody (Product # MA5-35547) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MTA2 in paraffin-embedded human colon. Samples were incubated with MTA2 Monoclonal antibody (Product # MA5-35547) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP analysis of MTA2 in extracts of HeLa cells. Samples were incubated with MTA2 Monoclonal antibody (Product # MA5-35547) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.