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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- 66195-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MTA2 Monoclonal Antibody (2G12G4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAANMYRVG DYVYFENSSS NPYLVRRIEE LNKTANGNVE AKVVCLFRRR DISSSLNSLA DSNAREFEEE SKQPGVSEQQ RHQLKHRELF LSRQFESLPA THIRGKCSVT LLNETDILSQ YLEKEDCFFY SLVFDPVQKT LLADQGEIRV GCKYQAEIPD RLVEGESDNR NQQKMEMKVW DPDNPLTDRQ IDQFLVVARA VGTFARALDC SSSIRQPSLH MSAAAASRDI TLFHAMDTLQ RNGYDLAKAM STLVPQGGPV LCRDEMEEWS ASEAMLFEEA LEKYGKDFND IRQDFLPWKS LASIVQFYYM WKTTDRYIQQ KRLKAAEADS KLKQVYIPTY TKPNPNQIIS V (1-350 aa encoded by BC053650)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2G12G4
- Vial size
- 150 µL
- Concentration
- 1.4 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 66195-1-IG ( MTA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 66195-1-Ig (MTA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 66195-1-IG (MTA2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IGG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66195-1-IG ( MTA2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66195-1-IG ( MTA2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with .2ug MTA2 antibody (66195-1-IG, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L) with dilution 1:1000.
