PA5-28432

antibody from Invitrogen Antibodies

Targeting: MTA2 MTA1-L1, MTA1L1

Antibody data

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Validation data

MTA2 protein structure - PA5-28432 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using MTA2 Polyclonal Antibody (Product # PA5-28432). Sample (30 µg of whole cell lysate). A: 293T. B: A431. 7.5% SDS PAGE. MTA2 Polyclonal Antibody (Product # PA5-28432) diluted at 1:1500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of MTA2 was achieved by transfecting HeLa with MTA2 specific siRNAs (Silencer® select Product # s17390, s17389). Western blot analysis (Fig. a) was performed using whole cell Lysates from the MTA2 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with MTA2 Polyclonal Antibody (Product # PA5-28432, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MTA2. An uncharacterized band at ~35 kD was also observed.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-MTA2 Polyclonal Antibody (Product # PA5-28432) and ~75 kDa band corresponding to MTA2 was observed in all the cell lines and tissues tested along with an uncharacterized band at ~34 kDa. Whole cell lysate (40ug lysate) of A-431 (Lane 1), IMR-32 (Lane 2) and HeLa (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The bot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).