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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00001748-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001748-M01, RRID:AB_538290
- Product name
- Mouse Monoclonal BP1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. DLX4 - distal-less homeobox 4
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references DLX4 is associated with orofacial clefting and abnormal jaw development.
Homeodomain protein DLX4 counteracts key transcriptional control mechanisms of the TGF-β cytostatic program and blocks the antiproliferative effect of TGF-β.
A homeobox gene related to Drosophila distal-less promotes ovarian tumorigenicity by inducing expression of vascular endothelial growth factor and fibroblast growth factor-2.
Wu D, Mandal S, Choi A, Anderson A, Prochazkova M, Perry H, Gil-Da-Silva-Lopes VL, Lao R, Wan E, Tang PL, Kwok PY, Klein O, Zhuan B, Slavotinek AM
Human molecular genetics 2015 Aug 1;24(15):4340-52
Human molecular genetics 2015 Aug 1;24(15):4340-52
Homeodomain protein DLX4 counteracts key transcriptional control mechanisms of the TGF-β cytostatic program and blocks the antiproliferative effect of TGF-β.
Trinh BQ, Barengo N, Naora H
Oncogene 2011 Jun 16;30(24):2718-29
Oncogene 2011 Jun 16;30(24):2718-29
A homeobox gene related to Drosophila distal-less promotes ovarian tumorigenicity by inducing expression of vascular endothelial growth factor and fibroblast growth factor-2.
Hara F, Samuel S, Liu J, Rosen D, Langley RR, Naora H
The American journal of pathology 2007 May;170(5):1594-606
The American journal of pathology 2007 May;170(5):1594-606
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BP1 Antibody (1F11) [H00001748-M01] - Analysis of DLX4 over-expressed 293 cell line, cotransfected with DLX4 Validated Chimera RNAi ( Cat # H00001748-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DLX4 monoclonal antibody (M01), clone 1F11 (Cat # H00001748-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BP1 Antibody (1F11) [H00001748-M01] - Analysis of DLX4 expression in transfected 293T cell line by DLX4 monoclonal antibody (M01), clone 1F11.Lane 1: DLX4 transfected lysate(26 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: BP1 Antibody (1F11) [H00001748-M01] - Detection limit for recombinant GST tagged DLX4 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: BP1 Antibody (1F11) [H00001748-M01] - Analysis of DLX4 transfected lysate using anti-DLX4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DLX4 MaxPab rabbit polyclonal antibody.
