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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA1-16602 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DLX4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The target sequence has 82% sequence homology with mouse.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of MDA-MB-231 cell lysates using Product # PA1-16602.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lanes: 1. BP1-specific band (36 kDa) from 10 µg of cell extract using anti-BP1 (1)antibody diluted 1:5,000 2. BP1-specific band after immunoprecipitation of 850 µg of cell extract using anti-BP1 (1) antibody diluted 1:1,000 3. Negative control for IP in lane 2: cell extract incubated with 50 µL of Protein A/G beads
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DLX4 in MDA-MB-231 cell lysates. Sample was incubated in DLX4 polyclonal antibody (Product # PA1-16602).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of DLX4 in breast cancer cells and normal breast tissue. Frozen samples were incubated in DLX4 polyclonal antibody (Product # PA1-16602). Top Panel: BP1 antibody detected in breast cancer cells. Bottom Panel: Staining of normal breast tissue.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of DLX4 was performed using cell extract. Antigen-antibody complexes were formed by incubating 850 µg cell extract with DLX4 polyclonal antibody (Product # PA1-16602) using a dilution 1:5,000. 2. BP1-specific band after immunoprecipitation (antibody diluted 1:1,000) 3. Negative control for IP in lane 2: cell extract incubated with 50 µL of Protein A/G beads.
