Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-56559 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DLX3 Recombinant Rabbit Monoclonal Antibody (JE64-45)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JE64-45
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of DLX3 with JAR cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp). Next they were incubated with DLX3 Recombinant Monoclonal Antibody (Product # MA5-56559)at a dilution of 1:100 in 1% BSA in PBST overnight at 4 ℃. Followed by Goat Anti-Rabbit IgG H&L at a dilution of 1:1,000. DAPI was used to stain the cell nuclei (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DLX3 with paraffin-embedded human skin tissue. The tissues were blocked in 1% BSA (20 min, room temp), washed with ddH2O and PBS, incubated with DLX3 Recombinant Monoclonal Antibody (Product # MA5-56559) at a dilution of 1:1,000 (1 hr, room temp).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of DLX3 with JAR cells. The cells were fixed, permeabilized and incubated with DLX3 Recombinant Monoclonal Antibody (Product # MA5-56559) at a dilution of 1:1,000 (red) and compared with Rabbit IgG Isotype Control (green). Followed by iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1:1,000 dilution (30 min, 4℃).
