Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 17-0494-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD49c (Integrin alpha 3) Monoclonal Antibody (P1B5), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This P1B5 monoclonal antibody reacts with human CD49c (also known as Integrin alpha 3). Integrins constitute a family of cell surface receptors that mediate adhesion between cells or between a cell and the extracellular matrix. These proteins are heterodimers consisting of two distinct subunits, alpha and beta. Integrins are expressed on hematopoietic, non-hematopoietic, and cancer cells. Integrin-mediated adhesion is regulated by changes in ligand affinity (or activation), cell shape, and integrin clustering and diffusion. Integrin alpha 3 forms a complex with the common beta 1 subunit to form the alpha3beta1 integrin. Studies have demonstrated the involvement of this integrin in wound healing and epithelial migration. The P1B5 monoclonal antibody has been reported to affect adhesion to type I and VI collagens, as well as fibronectin and laminin. Applications Reported: This P1B5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This P1B5 antibody has been pre-titrated and tested by flow cytometric analysis of HeLa cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- P1B5
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.
Alpha3beta1 integrin in epidermis promotes wound angiogenesis and keratinocyte-to-endothelial-cell crosstalk through the induction of MRP3.
Integrin activation takes shape.
Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes.
Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits.
Menon V, Thomas R, Elgueta C, Horl M, Osborn T, Hallett PJ, Bartos M, Isacson O, Pruszak J
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Alpha3beta1 integrin in epidermis promotes wound angiogenesis and keratinocyte-to-endothelial-cell crosstalk through the induction of MRP3.
Mitchell K, Szekeres C, Milano V, Svenson KB, Nilsen-Hamilton M, Kreidberg JA, DiPersio CM
Journal of cell science 2009 Jun 1;122(Pt 11):1778-87
Journal of cell science 2009 Jun 1;122(Pt 11):1778-87
Integrin activation takes shape.
Liddington RC, Ginsberg MH
The Journal of cell biology 2002 Sep 2;158(5):833-9
The Journal of cell biology 2002 Sep 2;158(5):833-9
Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes.
Wayner EA, Gil SG, Murphy GF, Wilke MS, Carter WG
The Journal of cell biology 1993 Jun;121(5):1141-52
The Journal of cell biology 1993 Jun;121(5):1141-52
Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits.
Wayner EA, Carter WG
The Journal of cell biology 1987 Oct;105(4):1873-84
The Journal of cell biology 1987 Oct;105(4):1873-84
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of HeLa cells with Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD49c (Integrin alpha 3) APC (purple histogram). Total viable cells were used for analysis.