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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- PA5-79528 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD49c (Integrin alpha 3) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: human U87 whole cell, human A431 whole cell, human A549 whole cell, human U2OS whole cell, rat C6 whole cell, rat PC-12 whole cell. IHC: human lung cancer tissue, human colorectal adenocarcinoma tissue. ICC/IF: A431 cell. Flow: U87 cell.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of CD49c (Integrin alpha 3) in A431 cells using CD49c (Integrin alpha 3) Polyclonal Antibody (Product # PA5-79528). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CD49c (Integrin alpha 3) on paraffin-embedded rat brain tissue. Sample was incubated with CD49c (Integrin alpha 3) polyclonal antibody (Product# PA5-79528).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CD49c (Integrin alpha 3) on paraffin-embedded human intestinal cancer tissue. Sample was incubated with CD49c (Integrin alpha 3) polyclonal antibody (Product# PA5-79528).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of CD49c (Integrin alpha 3) in paraffin-embedded section of human colorectal adenocarcinoma tissue using CD49c (Integrin alpha 3) Polyclonal Antibody (Product # PA5-79528). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilution overnight at 4°C. Peroxidase conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of CD49c (Integrin alpha 3) in paraffin-embedded section of human lung cancer tissue using CD49c (Integrin alpha 3) Polyclonal Antibody (Product # PA5-79528). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilution overnight at 4°C. Peroxidase conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD49c (Integrin alpha 3) in U87 cells using CD49c (Integrin alpha 3) Polyclonal Antibody (Product # PA5-79528), shown in overlay histogram (blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum, and incubated with the primary antibody (1 μg/1x10^6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
