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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- 66070-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Integrin alpha-3 Monoclonal Antibody (3F9G4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody can recognize the heavy chain.
- Antibody clone number
- 3F9G4
- Concentration
- 0.89 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- mouse brain tissue were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 66070-1-IG (ITGA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of ITGA3 antibody (66070-1-IG, 1:6000) with si-control and si-ITGA3 transfected A549 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66070-1-IG (Integrin alpha-3 antibody) at dilution of 1:4000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66070-1-IG (Integrin alpha-3 antibody) at dilution of 1:4000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 PC-3 cells were stained with 0.2 µg Anti-Human Integrin Alpha 3 (Product # 66070-1-IG, Clone: 3F9G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1,000 (green), or stained with 0.2 µg Mouse IgG2a Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1,000 (black). Cells were fixed with 90% MeOH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 A549 cells were stained with 0.2ug Integrin alpha-3 antibody (66070-1-IG, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L) with dilution 1:1500.
