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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Western blot [2]
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- Product number
- MAB9071-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MMP-7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects the pro and active forms of human MMP-7 in Western blots. In dot blots, approximately 20% cross-reactivity with recombinant human MMP-8 is observed and no cross-reactivity with recombinant human MMP-1, -2, -3, -9, -10, -12, or -13 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_002414.1- Isotype
- IgG
- Antibody clone number
- 111433
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references miR-126-3p down-regulation contributes to dabrafenib acquired resistance in melanoma by up-regulating ADAM9 and VEGF-A.
Coexpression of p-IGF-1R and MMP-7 Modulates Panitumumab and Cetuximab Efficacy in RAS Wild-Type Metastatic Colorectal Cancer Patients.
ATF3 reduces migration capacity by regulation of matrix metalloproteinases via NFκB and STAT3 inhibition in glioblastoma.
Integrin α5β1 facilitates cancer cell invasion through enhanced contractile forces.
Urinary matrix metalloproteinase-7 level is associated with the presence of metastasis in bladder cancer.
Matrix metalloproteinase-7 as a marker of metastasis and predictor of poor survival in bladder cancer.
Matrix metalloproteinase activity in pediatric acute lung injury.
Divergent functions for airway epithelial matrix metalloproteinase 7 and retinoic acid in experimental asthma.
Stable isotope dilution multidimensional liquid chromatography-tandem mass spectrometry for pancreatic cancer serum biomarker discovery.
Gingival fibroblast inhibits MMP-7: evaluation in an ex vivo aorta model.
IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
Caporali S, Amaro A, Levati L, Alvino E, Lacal PM, Mastroeni S, Ruffini F, Bonmassar L, Antonini Cappellini GC, Felli N, Carè A, Pfeffer U, D'Atri S
Journal of experimental & clinical cancer research : CR 2019 Jun 21;38(1):272
Journal of experimental & clinical cancer research : CR 2019 Jun 21;38(1):272
Coexpression of p-IGF-1R and MMP-7 Modulates Panitumumab and Cetuximab Efficacy in RAS Wild-Type Metastatic Colorectal Cancer Patients.
Alonso V, Escudero P, Fernández-Martos C, Salud A, Méndez M, Gallego J, Rodriguez JR, Martín-Richard M, Fernández-Plana J, Manzano H, Méndez JC, Zanui M, Falcó E, Gil-Raga M, Rojo F, Cuatrecasas M, Feliu J, García-Albéniz X, Maurel J
Neoplasia (New York, N.Y.) 2018 Jul;20(7):678-686
Neoplasia (New York, N.Y.) 2018 Jul;20(7):678-686
ATF3 reduces migration capacity by regulation of matrix metalloproteinases via NFκB and STAT3 inhibition in glioblastoma.
Guenzle J, Wolf LJ, Garrelfs NW, Goeldner JM, Osterberg N, Schindler CR, Saavedra JE, Weyerbrock A
Cell death discovery 2017;3:17006
Cell death discovery 2017;3:17006
Integrin α5β1 facilitates cancer cell invasion through enhanced contractile forces.
Mierke CT, Frey B, Fellner M, Herrmann M, Fabry B
Journal of cell science 2011 Feb 1;124(Pt 3):369-83
Journal of cell science 2011 Feb 1;124(Pt 3):369-83
Urinary matrix metalloproteinase-7 level is associated with the presence of metastasis in bladder cancer.
Szarvas T, Singer BB, Becker M, Vom Dorp F, Jäger T, Szendroi A, Riesz P, Romics I, Rübben H, Ergün S
BJU international 2011 Apr;107(7):1069-73
BJU international 2011 Apr;107(7):1069-73
Matrix metalloproteinase-7 as a marker of metastasis and predictor of poor survival in bladder cancer.
Szarvas T, Becker M, vom Dorp F, Gethmann C, Tötsch M, Bánkfalvi A, Schmid KW, Romics I, Rübben H, Ergün S
Cancer science 2010 May;101(5):1300-8
Cancer science 2010 May;101(5):1300-8
Matrix metalloproteinase activity in pediatric acute lung injury.
Kong MY, Gaggar A, Li Y, Winkler M, Blalock JE, Clancy JP
International journal of medical sciences 2009;6(1):9-17
International journal of medical sciences 2009;6(1):9-17
Divergent functions for airway epithelial matrix metalloproteinase 7 and retinoic acid in experimental asthma.
Goswami S, Angkasekwinai P, Shan M, Greenlee KJ, Barranco WT, Polikepahad S, Seryshev A, Song LZ, Redding D, Singh B, Sur S, Woodruff P, Dong C, Corry DB, Kheradmand F
Nature immunology 2009 May;10(5):496-503
Nature immunology 2009 May;10(5):496-503
Stable isotope dilution multidimensional liquid chromatography-tandem mass spectrometry for pancreatic cancer serum biomarker discovery.
Yu KH, Barry CG, Austin D, Busch CM, Sangar V, Rustgi AK, Blair IA
Journal of proteome research 2009 Mar;8(3):1565-76
Journal of proteome research 2009 Mar;8(3):1565-76
Gingival fibroblast inhibits MMP-7: evaluation in an ex vivo aorta model.
Gogly B, Fournier B, Couty L, Naveau A, Brasselet C, Durand E, Coulomb B, Lafont A
Journal of molecular and cellular cardiology 2009 Aug;47(2):296-303
Journal of molecular and cellular cardiology 2009 Aug;47(2):296-303
IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5957-65
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5957-65
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human MMP-7 by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and SK-OV-3 human ovarian adenocarcinoma cell line and, for additional reference, Recombinant Human MMP-7 Western Blot Standard Protein (Catalog # WBC016). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MMP-7 Monoclonal Antibody (Catalog # MAB9071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human MMP-7 by Simple WesternTM. Simple Western lane view shows lysates of SK-OV-3 human ovarian adenocarcinoma cell line, human small intestine tissue, and CaSki human cervical carcinoma, loaded at 0.5 mg/mL. Specific bands were detected for MMP-7 at approximately 49 and 65 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human MMP-7 Monoclonal Antibody (Catalog # MAB9071) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
