PA5-119931

antibody from Invitrogen Antibodies

Targeting: MMP7 MPSL1, PUMP-1

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-119931 - Provider product page
Provider: Invitrogen Antibodies
Product name: MMP7 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Positive Control: A549 cell lysates, HT-29 cell lysate, A431 cell lysate, A549, human uterus tissue, HT-29. Subcellular Location: Extracellular matrix, Secreted.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

MMP7 protein structure - PA5-119931 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of of MMP7 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with MMP7 Polyclonal Antibody (Product # PA5-119931) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of of MMP7 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with MMP7 Polyclonal Antibody (Product # PA5-119931) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of of MMP7 in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with MMP7 Polyclonal Antibody (Product # PA5-119931) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).