PA5-93140

antibody from Invitrogen Antibodies

Targeting: NPHP1 JBTS4, NPH1, SLSN1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-93140

Provider

Invitrogen Antibodies

Product name

NPHP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PDLGILFELG ISYIRNSTGE RGELSCGWVF LKLFDASGVP IPAKTYELFL NGGTPYEKGI EVDPSISRRA HGSVFYQIMT MRRQPQLLVK LRSLNRRSRN VLSLLPETLI GNMCSIHLLI FYRQILGDVL LKDRMSLQST DLISHPMLAT FPMLLEQPDV MDALRSSWAG KESTLKRSEK RDKEFLKSTF LLVYHDCVLP LLHSTRLPPF RWAEEETETA RWKVITDFLK QNQENQGALQ ALLSPDGVHE PFDLSEQTYD FLGEMRKNAV; Positive Samples: Mouse lung, Rat brain; Cellular Location: Cell junction, Cell projection, Cytoplasm, adherens junction, cilium, cilium axoneme, cytoskeleton, tight junction

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.97 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of NPHP1 was performed in MCF7 cells using NPHP1 Polyclonal Antibody (Product # PA5-93140).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of NPHP1 in NIH/3T3 cells. Samples were incubated with NPHP1 Polyclonal antibody (Product # PA5-93140) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of NPHP1 in PC-12 cells. Samples were incubated with NPHP1 Polyclonal antibody (Product # PA5-93140) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of NPHP1 in U2OS cells. Samples were incubated with NPHP1 Polyclonal antibody (Product # PA5-93140) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NPHP1 in paraffin-embedded rat testis. Samples were incubated with NPHP1 Polyclonal antibody (Product # PA5-93140) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NPHP1 in paraffin-embedded rat kidney. Samples were incubated with NPHP1 Polyclonal antibody (Product # PA5-93140) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.