Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 675702 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#675702, RRID:AB_2565606
- Product name
- Purified anti-MMP1
- Antibody type
- Monoclonal
- Antigen
- Recombinant human MMP-1.
- Description
- Members of the matrix metalloproteinase family proteins (MMPs) are structurally related and zinc-containing enzymes that not only degrade the extracellular matrix (ECM) and connective tissue proteins under normal physiological setting but also during disease (e.g. arthritis and metastasis). The MMP family includes six different groups of enzymes: collagenases, gelatinases, stromelysins, transmembrane MMPs, matrilysins and others. MMPs are secreted as proenzymes that have to be cleaved in order to be activated. MMP1 consists of a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site, a short hinge region, and a carboxyl terminal (hemopexin-like repeats) domain. It is thought to play an important role in rheumatoid and osteo-arthritis pathogenesis as mediators of cartilage and bone destruction. Substrates of MMP-1 include collagen I, II, III, VII, VIII, and X as well as casein, gelatin, alpha1 antitrypsin, myelin basic protein, L Selectin, pro-TNF, IL-1β, IGFBP3, IGFBP5, pro-MMP-2, and pro-MMP-9. In addition, chemokines are substrates for MMP-1. CCL2, CCL7, CCL8, and CCL13 are catalytically cleaved by MMP-1 to produce receptor antagonists. CXCL5 and CXCL12 are also substrates for MMP1. TIMPs inhibit MMPs in a 1:1 inhibitor to enzyme ratio through interaction of the N-terminal domain of the TIMP molecule with the active site of the MMP. MMP1 is overexpressed in invasive melanoma, colorectal, and esophageal cancers. Importantly, MMP-1 expression was shown to increase progressively with tumor stage.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- M4110F03
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Recombinant human MMP1 (Cat. No. 592902; 50 ng) treated with (lane 2) and without (lane 1) active MMP3 were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-MMP1 (clone M4110F03) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306) and chemiluminescence detection.
- Conjugate
- Unconjugated