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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- MA5-27653 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KCNQ4 Monoclonal Antibody (N43/6)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- 1 µg/mL of MA5-27653 was sufficient for detection of KCNQ4 in 10 µg of COS-1 cell lysate transiently expressing KCNQ4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 77kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- N43/6
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KCNQ4 in rat tissue lysate with 15 µg of sample. The sample was blocked with 1.5% BSA, incubated with KCNQ4 monoclonal antibody (Product # MA5-27653) using a dilution of 1:1000 (2 hours at 4°C), followed by Sheep Anti-Mouse HRP (1 hour at RT).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KCNQ4 in mouse frozen brain section. Samples were incubated with KCNQ4 monoclonal antibody (Product # MA5-27653) using a dilution of 1:1000 (1 hour at RT) and followed by Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) and Mayer Hematoxylin (purple/blue) at a dilution of 250-500 µl.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KCNQ4 in paraffin-embedded human hippocampus. Sample was fixed with Bouin's Fixative Solution, incubated with KCNQ4 monoclonal antibody (Product # MA5-27653) using a dilution of 1:1000 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) (1 hour at RT) at a dilution of 1:50.
