Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 680402 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#680402, RRID:AB_2566356
- Product name
- Purified anti-IGF1
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human IGF-I, amino acids (Gly49-Ala118) (Accession# PO05019), expressed in E. coli
- Description
- IGF-I, also known as insulin-like growth factor 1), was initially described in 1957 as a hormone that is important for child development, and also has anabolic effects in adults. It is a member of the IGF family that is comprised of three members: IGF-I, IGF-II, and insulin. IGF-I and IGF-II share approximately 50% similarity with insulin at the amino acid level. Human IGF-I is synthesized as a 191 amino acid prepro-IGF-I; pro-IGF-I is processed to the mature, active protein, and this procedure requires glucose regulating protein 94 (GRP94) that possesses chaperone activity towards IGF-I. Deletion of muscle GRP94 impairs muscle and body growth by inhibiting local production of IGF proteins. Seven IGF binding proteins have been described (IGFBP-1 to -7). IGFBPs extend the half-lives and regulate the availability of IGF-I and IGF-II. It has been described that IGFBP3 is the main circulating binding protein for IGFs in serum. 75% of circulating IGF-I and IGF-II bind to IGFBP3 and the co-carrier acid labile subunit (ALS). IGF-I binds to three receptors, and the binding to IGFIR promotes cell proliferation, survival, differentiation, and migration. IGF-I deficiency is associated with growth hormone insensitivity syndrome (GHIS), which is also called Laron syndrome, liver cirrhosis, age-related cardiovascular, neurological diseases, and intrauterine growth restriction.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 1500102
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Recombinant human IGF-I (50ng) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-IGF1 (clone 1500102) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated