PA5-27207

antibody from Invitrogen Antibodies

Targeting: IGF1 IGF, IGF-I, IGF1A, IGFI

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27207

Provider

Invitrogen Antibodies

Product name

IGF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: PC-3, U87-MG, SK-N-SH. Predicted reactivity: Mouse (93%), Rat (94%), Xenopus laevis (80%), Dog (98%), Pig (96%), Rabbit (98%), Chicken (85%), Sheep (96%), Rhesus Monkey (100%), Bovine (97%), Guinea pig (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.16 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Translation of Tudor-SN, a novel terminal oligo-pyrimidine (TOP) mRNA, is regulated by the mTORC1 pathway in cardiomyocytes.

Gan S, Su C, Ma J, Liu M, Cui X, Xin L, Ren Y, Gao X, Ge L, Wei M, Yang J
RNA biology 2021 Jun;18(6):900-913

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Innovative Three-Dimensional Microscopic Analysis of Uremic Growth Plate Discloses Alterations in the Process of Chondrocyte Hypertrophy: Effects of Growth Hormone Treatment.

Fernández-Iglesias Á, Fuente R, Gil-Peña H, Alonso-Durán L, García-Bengoa M, Santos F, López JM
International journal of molecular sciences 2020 Jun 25;21(12)

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The role of miR-24 as a race related genetic factor in prostate cancer.

Hashimoto Y, Shiina M, Kato T, Yamamura S, Tanaka Y, Majid S, Saini S, Shahryari V, Kulkarni P, Dasgupta P, Mitsui Y, Sumida M, Deng G, Tabatabai L, Kumar D, Dahiya R
Oncotarget 2017 Mar 7;8(10):16581-16593

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Differential expression of miR-34b and androgen receptor pathway regulate prostate cancer aggressiveness between African-Americans and Caucasians.

Shiina M, Hashimoto Y, Kato T, Yamamura S, Tanaka Y, Majid S, Saini S, Varahram S, Kulkarni P, Dasgupta P, Mitsui Y, Sumida M, Tabatabai L, Deng G, Kumar D, Dahiya R
Oncotarget 2017 Jan 31;8(5):8356-8368

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IGF1 Polyclonal Antibody detects IGF1 protein at Golgi apparatus membrane by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF1 protein stained by IGF1 Polyclonal Antibody (Product # PA5-27207) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 miR-34b target genes and luciferase reporter assay A. qPCR analysis of AR, BCL2, ETV1, PDPK1 and IGF1 from MDA-PCa-2b or DU-145 cells transfected with miR-34b-3p mimic. Asterisks indicate a p-value

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 Predicted target gene expression changes after miR-24 induction ( A ) qPCR analyses for the predicted target genes of miR-24. We checked candidate gene expression levels after miR-24 over-expression. * p < 0.05, Bar = +- SEM. ( B ) Western blotting of miR-24 target genes. ( C )The results of Dual Luciferase Reporter Assays. Except for IGF1, all genes have two different binding sites (for instance; AR-1 and AR-2 mean that miR-24 was predicted that it may bind to two different site of AR mRNA). * p < 0.05, n.s; no significance, Bar = +- SEM. ( D ) Linear regression model of mRNA expression levels between miR-24-1 and its five target genes in human PCa samples.