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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [21]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-291-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human IGF-I/IGF-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IGF-I/IGF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IGF-I/IGF-1 and recombinant rat IGF-I/IGF-1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 250 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Therapeutic Effects of rAAV-Mediated Concomittant Gene Transfer and Overexpression of TGF-β and IGF-I on the Chondrogenesis of Human Bone-Marrow-Derived Mesenchymal Stem Cells.
The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells.
Dopamine neuron-derived IGF-1 controls dopamine neuron firing, skill learning, and exploration.
The effect of cinnamon on polycystic ovary syndrome in a mouse model.
Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma.
Protein C receptor stimulates multiple signaling pathways in breast cancer cells.
Peripheral blood aspirates overexpressing IGF-I via rAAV gene transfer undergo enhanced chondrogenic differentiation processes.
Adipose stem cells from chronic pancreatitis patients improve mouse and human islet survival and function.
Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I.
A subset of bone marrow stromal cells regulate ATP-binding cassette gene expression via insulin-like growth factor-I in a leukemia cell line.
Hypoxia enhances protective effect of placental-derived mesenchymal stem cells on damaged intestinal epithelial cells by promoting secretion of insulin-like growth factor-1.
IGF-1 promotes the development and cytotoxic activity of human NK cells.
Benefits of recombinant adeno-associated virus (rAAV)-mediated insulinlike growth factor I (IGF-I) overexpression for the long-term reconstruction of human osteoarthritic cartilage by modulation of the IGF-I axis.
Growth hormone promotes lymphangiogenesis.
Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
Effect of construct properties on encapsulated chondrocyte expression of insulin-like growth factor-1.
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells.
Gene induction during differentiation of human pulmonary type II cells in vitro.
Mining the acute respiratory distress syndrome proteome: identification of the insulin-like growth factor (IGF)/IGF-binding protein-3 pathway in acute lung injury.
Quantification, localization, and expression of IGF-I and TGF-beta1 during growth factor-stimulated fracture healing.
Morscheid S, Rey-Rico A, Schmitt G, Madry H, Cucchiarini M, Venkatesan JK
International journal of molecular sciences 2019 May 27;20(10)
International journal of molecular sciences 2019 May 27;20(10)
The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells.
Pakuła M, Mikuła-Pietrasik J, Witucka A, Kostka-Jeziorny K, Uruski P, Moszyński R, Naumowicz E, Sajdak S, Tykarski A, Książek K
International journal of molecular sciences 2019 Jan 2;20(1)
International journal of molecular sciences 2019 Jan 2;20(1)
Dopamine neuron-derived IGF-1 controls dopamine neuron firing, skill learning, and exploration.
Pristerà A, Blomeley C, Lopes E, Threlfell S, Merlini E, Burdakov D, Cragg S, Guillemot F, Ang SL
Proceedings of the National Academy of Sciences of the United States of America 2019 Feb 26;116(9):3817-3826
Proceedings of the National Academy of Sciences of the United States of America 2019 Feb 26;116(9):3817-3826
The effect of cinnamon on polycystic ovary syndrome in a mouse model.
Dou L, Zheng Y, Li L, Gui X, Chen Y, Yu M, Guo Y
Reproductive biology and endocrinology : RB&E 2018 Oct 19;16(1):99
Reproductive biology and endocrinology : RB&E 2018 Oct 19;16(1):99
Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma.
Nagel S, Pommerenke C, Meyer C, Kaufmann M, MacLeod RAF, Drexler HG
Oncotarget 2018 Mar 6;9(18):14338-14353
Oncotarget 2018 Mar 6;9(18):14338-14353
Protein C receptor stimulates multiple signaling pathways in breast cancer cells.
Wang D, Liu C, Wang J, Jia Y, Hu X, Jiang H, Shao ZM, Zeng YA
The Journal of biological chemistry 2018 Jan 26;293(4):1413-1424
The Journal of biological chemistry 2018 Jan 26;293(4):1413-1424
Peripheral blood aspirates overexpressing IGF-I via rAAV gene transfer undergo enhanced chondrogenic differentiation processes.
Frisch J, Orth P, Rey-Rico A, Venkatesan JK, Schmitt G, Madry H, Kohn D, Cucchiarini M
Journal of cellular and molecular medicine 2017 Nov;21(11):2748-2758
Journal of cellular and molecular medicine 2017 Nov;21(11):2748-2758
Adipose stem cells from chronic pancreatitis patients improve mouse and human islet survival and function.
Song L, Sun Z, Kim DS, Gou W, Strange C, Dong H, Cui W, Gilkeson G, Morgan KA, Adams DB, Wang H
Stem cell research & therapy 2017 Aug 30;8(1):192
Stem cell research & therapy 2017 Aug 30;8(1):192
Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I.
Frisch J, Rey-Rico A, Venkatesan JK, Schmitt G, Madry H, Cucchiarini M
Tissue engineering. Part A 2015 Sep;21(17-18):2460-71
Tissue engineering. Part A 2015 Sep;21(17-18):2460-71
A subset of bone marrow stromal cells regulate ATP-binding cassette gene expression via insulin-like growth factor-I in a leukemia cell line.
Benabbou N, Mirshahi P, Bordu C, Faussat AM, Tang R, Therwath A, Soria J, Marie JP, Mirshahi M
International journal of oncology 2014 Oct;45(4):1372-80
International journal of oncology 2014 Oct;45(4):1372-80
Hypoxia enhances protective effect of placental-derived mesenchymal stem cells on damaged intestinal epithelial cells by promoting secretion of insulin-like growth factor-1.
Du L, Yu Y, Ma H, Lu X, Ma L, Jin Y, Zhang H
International journal of molecular sciences 2014 Jan 27;15(2):1983-2002
International journal of molecular sciences 2014 Jan 27;15(2):1983-2002
IGF-1 promotes the development and cytotoxic activity of human NK cells.
Ni F, Sun R, Fu B, Wang F, Guo C, Tian Z, Wei H
Nature communications 2013;4:1479
Nature communications 2013;4:1479
Benefits of recombinant adeno-associated virus (rAAV)-mediated insulinlike growth factor I (IGF-I) overexpression for the long-term reconstruction of human osteoarthritic cartilage by modulation of the IGF-I axis.
Weimer A, Madry H, Venkatesan JK, Schmitt G, Frisch J, Wezel A, Jung J, Kohn D, Terwilliger EF, Trippel SB, Cucchiarini M
Molecular medicine (Cambridge, Mass.) 2012 May 9;18(1):346-58
Molecular medicine (Cambridge, Mass.) 2012 May 9;18(1):346-58
Growth hormone promotes lymphangiogenesis.
Banziger-Tobler NE, Halin C, Kajiya K, Detmar M
The American journal of pathology 2008 Aug;173(2):586-97
The American journal of pathology 2008 Aug;173(2):586-97
Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
Skibinski G, Elborn JS, Ennis M
American journal of physiology. Lung cellular and molecular physiology 2007 Jul;293(1):L69-76
American journal of physiology. Lung cellular and molecular physiology 2007 Jul;293(1):L69-76
Effect of construct properties on encapsulated chondrocyte expression of insulin-like growth factor-1.
Yoon DM, Hawkins EC, Francke-Carroll S, Fisher JP
Biomaterials 2007 Jan;28(2):299-306
Biomaterials 2007 Jan;28(2):299-306
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
Gastroenterology 2006 May;130(6):1754-63
Gastroenterology 2006 May;130(6):1754-63
Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells.
Lee JH, Ryu IH, Kim EK, Lee JE, Hong S, Lee HK
Investigative ophthalmology & visual science 2006 Mar;47(3):864-72
Investigative ophthalmology & visual science 2006 Mar;47(3):864-72
Gene induction during differentiation of human pulmonary type II cells in vitro.
Wade KC, Guttentag SH, Gonzales LW, Maschhoff KL, Gonzales J, Kolla V, Singhal S, Ballard PL
American journal of respiratory cell and molecular biology 2006 Jun;34(6):727-37
American journal of respiratory cell and molecular biology 2006 Jun;34(6):727-37
Mining the acute respiratory distress syndrome proteome: identification of the insulin-like growth factor (IGF)/IGF-binding protein-3 pathway in acute lung injury.
Schnapp LM, Donohoe S, Chen J, Sunde DA, Kelly PM, Ruzinski J, Martin T, Goodlett DR
The American journal of pathology 2006 Jul;169(1):86-95
The American journal of pathology 2006 Jul;169(1):86-95
Quantification, localization, and expression of IGF-I and TGF-beta1 during growth factor-stimulated fracture healing.
Wildemann B, Schmidmaier G, Brenner N, Hüning M, Stange R, Haas NP, Raschke M
Calcified tissue international 2004 Apr;74(4):388-97
Calcified tissue international 2004 Apr;74(4):388-97
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IGF-I/IGF-1 in MDA-MB-231 Human Cell Line. IGF-I/IGF-1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IGF-I/IGF-1 in Human Placenta. IGF-I/IGF-1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Human IGF-I/IGF-1 Antibody. Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). The ND50 is typically 3-12 µg/mL.
