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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AP11883PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP11883PU-N, RRID:AB_1748817
- Product name
- anti ASCL1 (N-term)
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Hash1.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- The anti-Hash1 Antibody (N-term) (AP11883PU-N) is used in Western blot to detect Hash1 in mouse lung tissue lysate.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Hash1 (arrow) using rabbit polyclonal Hash1 Antibody (N-term) (AP11883PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the Hash1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
