PA5-40527

antibody from Invitrogen Antibodies

Targeting: ASCL1 ASH1, bHLHa46, HASH1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-40527

Provider

Invitrogen Antibodies

Product name

ASCL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: QSAQQQQQQQ QQQQQAPQLR PAADGQPSGG GHKSAPKQVK RQRSSSPELM

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-ASCL1 Polyclonal Antibody (Product # PA5-40527) and a 25 kDa band corresponding to Ascl1 was observed across cell lines tested. Nuclear enriched extracts (30 µg lysate) of PC-12 (Lane 1), Neuro-2a (Lane 2) in Fig. a; SH-SY5Y (Lane 1), iPSC(Lane 2), NSC (Lane 3), NSC differentiated to neurons (Lane 4), SK-N-AS (Lane 5) in Fig. b were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human thymus cells using an anti-ASCL1 polyclonal antibody (Product # PA5-40527).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of human kidney tissue using an anti-ASCL1 polyclonal antibody (Product # PA5-40527).