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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- 600-101-MH4S - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Mash1/Ascl1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of Goat Anti-Mash1/Ascl1 Antibody. Lane 1: Opal Pre-Stained Molecular Weight Marker (p/n MB-210-0500). Lane 2: MASH1/ASCL1 Human recombinant protein C-MYC/DDK-tagged (50ng) [+]. Primary Antibody: Anti-Mash1/Ascl1 at 1:1000 overnight at 2-8°C. Secondary Antibody: Donkey Fab2 Anti-Goat H&L mx8 HRP at 1:5000 at RT for 1hr. Blocking Buffer: BlockOut® - Universal Blocking Buffer for Western Blotting (p/n MB-073). Expect: 24.7kDa, ~37kDa recombinant.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of Goat Anti-Mash1/Ascl1 Antibody. Opal Pre-Stained Molecular Weight Marker (p/n MB-210-0500). Lane 1: Mouse Pancreas (35µg) (p/n W10-000-T023) [-]. Lane 2: SH-SY5Y whole cell lysate (35µg) [+]. Primary Antibody: Anti-Mash1/Ascl1 at 1:1000 overnight at 2-8°C. Secondary Antibody: Rabbit Anti-Goat IgG HRP (p/n 605-4313) at 1:5000 at RT for 1hr. Blocking Buffer: BlockOut® - Universal Blocking Buffer for Western Blotting (p/n MB-073). Expect: 24.7kDa, observed ~37kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of Goat Anti-Mash1/Ascl1 Antibody. Tissue: Human Thyroid Papillary Carcinoma Tissue. Fixative: none. Antigen Retrieval: HEIR pH6. Primary Antibody: Anti-Mash1/Ascl1 at 1:1,000. Counterstain: Hematoxylin. The Abs developed intense nucleus staining in human thyroid papillary cancer tissue.
