TA319499

antibody from OriGene

Targeting: ANAPC1 APC1, MCPR, TSG24

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319499

Provider

OriGene

Product name

Rabbit polyclonal APC1 phospho S355 antibody (Phospho-specific)

Antibody type

Polyclonal

Description

Rabbit polyclonal APC1 phospho S355 antibody (Phospho-specific)

Reactivity

Canine

Host

Rabbit

Conjugate

Unconjugated

Epitope

ANAPC1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

1.0 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB using Anti-APC1 pS355 antibody shows detection of a band ~215 kDa corresponding to phosphorylated human APC1 (arrowhead). Lane 1:lysate from asynchronous cells. Lane 2: lysate from cells treated with thymidine to synchronize cells at the G1/S boundary. Lane 3:lysate from cells treated with nocodazole to synchronize cells at the M phase. Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division. Primary antibody was used at 1:500.

Validation comment

WB