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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NB100-2244 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2244, RRID:AB_2157197
- Product name
- Rabbit Polyclonal NUP214 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Metabolic labeling enables selective photocrosslinking of O-GlcNAc-modified proteins to their binding partners.
Yu SH, Boyce M, Wands AM, Bond MR, Bertozzi CR, Kohler JJ
Proceedings of the National Academy of Sciences of the United States of America 2012 Mar 27;109(13):4834-9
Proceedings of the National Academy of Sciences of the United States of America 2012 Mar 27;109(13):4834-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NUP214 Antibody [NB100-2244] - Detection of Human NUP214 on HeLa whole cell lysate using NB100-2244. NUP214 was also immunoprecipitated using rabbit anti-NUP214 antibody BL2244 NB100-2245.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NUP214 Antibody [NB100-2244] - Whole cell lysate from HeLa (5, 15 and 50 ug), and HEK293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP214 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: NUP214 Antibody [NB100-2244] - Affinity purified rabbit anti-NUP214 (NB100-2244) used at a dilution of 1:100 (2ug/ml). Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated used at a dilution of 1:100.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NUP214 Antibody [NB100-2244] - Detection of human NUP214 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NUP214 antibody NB100-2244 (lot 2) used for IP at 6 ug per reaction. NUP214 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-NUP214 antibody NB100-2245. For blotting immunoprecipitated NUP214, NB100-2244 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NUP214 Antibody [NB100-2244] - Section of ovarian carcinoma. Antibody: Affinity purified rabbit anti- NUP214 used at a dilution of 1:200 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NUP214 Antibody [NB100-2244] - Section of ovarian carcinoma. Antibody: Affinity purified rabbit anti- NUP214 used at a dilution of 1:200 (1ug/ml). Detection: DAB
