HPA041141

antibody from Atlas Antibodies

Targeting: HIF3A bHLHe17, IPAS, MOP7, PASD7

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA041141

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA041141, RRID:AB_10803997

Product name

Anti-HIF3A

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human HIF3A, Gene description: hypoxia inducible factor 3, alpha subunit, Alternative Gene Names: bHLHe17, IPAS, MOP7, PASD7, Validated applications: IHC, ChIP, Uniprot ID: Q9Y2N7, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.4 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Hypoxia Signaling Cascade for Erythropoietin Production in Hepatocytes

Tojo Y, Sekine H, Hirano I, Pan X, Souma T, Tsujita T, Kawaguchi S, Takeda N, Takeda K, Fong G, Dan T, Ichinose M, Miyata T, Yamamoto M, Suzuki N
Molecular and Cellular Biology 2023;35(15):2658-2672

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

ChIP-Exo-Seq composite graph for Anti-HIF3A (HPA041141, Lot A118930) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.