Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA800720 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA800720, RRID:AB_2625638
- Product name
- HIF3A (HIF3 alpha) mouse monoclonal antibody, clone OTI2D2 (formerly 2D2)
- Antibody type
- Monoclonal
- Description
- HIF3A (HIF3 alpha) mouse monoclonal antibody, clone OTI2D2 (formerly 2D2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HIF3A
- Isotype
- IgG
- Antibody clone number
- OTI2D2
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references Pro-inflammatory cytokines activate hypoxia-inducible factor 3α via epigenetic changes in mesenchymal stromal/stem cells.
Cuomo F, Coppola A, Botti C, Maione C, Forte A, Scisciola L, Liguori G, Caiafa I, Ursini MV, Galderisi U, Cipollaro M, Altucci L, Cobellis G
Scientific reports 2018 Apr 11;8(1):5842
Scientific reports 2018 Apr 11;8(1):5842
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIF3A (RC210255, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIF3A.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HIF3A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800720)
- Validation comment
- IHC