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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- RQ5626 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- HIF3A Antibody / Hypoxia-inducible factor 3 alpha
- Antibody type
- Polyclonal
- Description
- This highly specific HIF3A antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the HIF3A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat lung, 2) rat skeletal muscle, 3) mouse lung and 4) mouse NIH 3T3 lysate with HIF3A antibody. Expected molecular weight ~72 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) A431, 3) Caco-2, 4) U-87 MG, 5) U-2 OS, 6) K562 and 7) PC-3 lysate with HIF3A antibody. Expected molecular weight ~72 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-87 MG cells with HIF3A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HIF3A antibody.
