Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- R1575P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1575P, RRID:AB_1002380
- Product name
- anti HIF3A / HIF3 alpha (581-592)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to amino acids 581-592 of Mouse HIF3A.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 1.2 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using Purified anti-Hif3 α antibody shows detection of a band ~72 kDa corresponding to mouse Hif3a (arrowhead). Approximately 10 µg of a CoCl2 treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. Treatment of exponentially growing 3T3 cells with 130 µM CoCl2 for 6 h at 37°C effectively mimicshypoxia. After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:1,600. The membrane was washed and reacted with a 1:10,000 dilution of IRDyeTM 800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature. IRDyeTM 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-Fixed, Paraffin-Embedded human lung, respiratory epithelium tissue stained with HIF3A Antibody Cat.-No R1575P after heat-induced antigen retrieval.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-Fixed, Paraffin-Embedded human Placenta tissue stained with HIF3A Antibody Cat.-No R1575P after heat-induced antigen retrieval.