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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX128146 - Provider product page
- Provider
- GeneTex
- Product name
- ATR antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ATR antibody detects ATR protein by Western blot analysis.A. 30 µg A431 whole cell lysate/extract5 % SDS-PAGEATR antibody (GTX128146) dilution: 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ATR antibody detects ATR protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody (GTX128146) diluted at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ATR antibody detects ATR protein by western blot analysis. Raji whole cell extracts and nuclear extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody (GTX128146) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Raji whole cell and nuclear extracts (30 ?g) were separated by 5% SDS-PAGE, and the membranes were blotted with ATR antibody (GTX128146) diluted at 1:500 and competitor's antibody (CST#2790) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Raji whole cell and nuclear extracts (30 ?g) were separated by 5% SDS-PAGE, and the membranes were blotted with ATR antibody (GTX128146) diluted at 1:500 and competitor's antibody (CST#2790) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ATR antibody detects ATR protein at nucleolus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATR protein stained by ATR antibody (GTX128146) diluted at 1:5000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of ATR protein from HCT-116 whole cell extracts treated with Hydroxyurea for 6 hr using 5 £gg of ATR antibody (GTX128146).Western blot analysis was performed using ATR antibody (GTX128146).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ATR antibody detects ATR protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. ATR antibody (GTX128146) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ATR antibody detects ATR protein at nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. ATR antibody (GTX128146) diluted at 1:250.
