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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- MA1-23158 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATR Monoclonal Antibody (2B5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji, Raji nuclear extract. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2B5
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Chk1 inhibitor synergizes quinacrine mediated apoptosis in breast cancer cells by compromising the base excision repair cascade.
Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA.
Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA.
Preet R, Siddharth S, Satapathy SR, Das S, Nayak A, Das D, Wyatt MD, Kundu CN
Biochemical pharmacology 2016 Apr 1;105:23-33
Biochemical pharmacology 2016 Apr 1;105:23-33
Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA.
Wilkinson VL, Warrier RR, Truitt TP, Nunes P, Gately MK, Presky DH
Journal of immunological methods 1996 Jan 16;189(1):15-24
Journal of immunological methods 1996 Jan 16;189(1):15-24
Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA.
Wilkinson VL, Warrier RR, Truitt TP, Nunes P, Gately MK, Presky DH
Journal of immunological methods 1996 Jan 16;189(1):15-24
Journal of immunological methods 1996 Jan 16;189(1):15-24
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATR in Raji whole cell and nuclear extracts (30 µg). Samples were separated by 5% SDS-PAGE and the membrane was probed with ATR Monoclonal antibody (Product # MA1-23158) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ATR was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ATR Monoclonal Antibody (2B5) (Product # MA1-23158) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ATR Monoclonal Antibody (2B5) (Product # MA1-23158). Raji whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR Polyclonal Antibody ATR Monoclonal Antibody (2B5) (Product # MA1-23158) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of ATM (Anti-ATM 2C1, (Product # MA1-23152) or ATR (Anti-ATR 2B5,ATR Monoclonal Antibody (2B5) (Product # MA1-23158) by western blot in human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa, using ATR (Product # MA1-23158) antibody at 1:100 dilution.
