LS-C60116

antibody from LSBio

Targeting: ATR FRP1, MEC1, SCKL, SCKL1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

LS-C60116

Provider

LSBio

Product name

ATR Antibody LS-C60116

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat, Canine, Simian, Xenopus

Host

Rabbit

Isotype

IgG

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-ATR Antibody - Western Blot. Western blot of anti-ATR antibody shows detection of ATR in HeLa cell nuclear extract (lane 1). Lane 2 shows negligible staining after pre-incubation of antibody with the immunizing peptide (50 ug peptide for 1 h at room temperature followed by centrifugation). A 4-8% gradient gel was used for separation. Goat serum was used at 5% for blocking. The arrowhead corresponds to 301 kD ATR when compared to MW markers (Lane M). The primary antibody was used at a 1:1400 dilution.