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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [25]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- GTX70109 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX70109, RRID:AB_368158
- Product name
- ATR antibody [2B5]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
Submitted references Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks.
Melatonin Sensitizes Hepatocellular Carcinoma Cells to Chemotherapy Through Long Non-Coding RNA RAD51-AS1-Mediated Suppression of DNA Repair.
TP53 Haploinsufficiency Rescues Emergency Granulopoiesis in FANCC-/- Mice.
The anticancer effects of MPT0G211, a novel HDAC6 inhibitor, combined with chemotherapeutic agents in human acute leukemia cells.
Checkpoint kinase 2 is dispensable for regulation of the p53 response but is required for G2/M arrest and cell survival in cells with p53 defects under heat stress.
RPA inhibition increases replication stress and suppresses tumor growth.
RAD50 phosphorylation promotes ATR downstream signaling and DNA restart following replication stress.
Ataxia telangiectasia-mutated- and Rad3-related protein regulates the DNA damage-induced G2/M checkpoint through the Aurora A cofactor Bora protein.
Inhibition of checkpoint kinase 1 abrogates G2/M checkpoint activation and promotes apoptosis under heat stress.
Hsp90 inhibitor-mediated disruption of chaperone association of ATR with hsp90 sensitizes cancer cells to DNA damage.
Nek1 kinase functions in DNA damage response and checkpoint control through a pathway independent of ATM and ATR.
Nek1 kinase functions in DNA damage response and checkpoint control through a pathway independent of ATM and ATR.
ATM- and ATR-mediated response to DNA damage induced by a novel camptothecin, ST1968.
Bifunctional DNA alkylator 1,3-bis(2-chloroethyl)-1-nitrosourea activates the ATR-Chk1 pathway independently of the mismatch repair pathway.
Interaction between PARP-1 and ATR in mouse fibroblasts is blocked by PARP inhibition.
Differential roles of ATR and ATM in p53, Chk1, and histone H2AX phosphorylation in response to hyperoxia: ATR-dependent ATM activation.
Ataxia-telangiectasia and Rad3-related and DNA-dependent protein kinase cooperate in G2 checkpoint activation by the DNA strand-breaking nucleoside analogue 2'-C-cyano-2'-deoxy-1-beta-D-arabino-pentofuranosylcytosine.
Caffeine yields aneuploidy through asymmetrical cell division caused by misalignment of chromosomes.
ATR-dependent checkpoint modulates XPA nuclear import in response to UV irradiation.
NBS1 mediates ATR-dependent RPA hyperphosphorylation following replication-fork stall and collapse.
DNA damage responses in progeroid syndromes arise from defective maturation of prelamin A.
Phosphorylation of nucleotide excision repair factor xeroderma pigmentosum group A by ataxia telangiectasia mutated and Rad3-related-dependent checkpoint pathway promotes cell survival in response to UV irradiation.
Regulation of CHK2 by DNA-dependent protein kinase.
Hypoxia-induced phosphorylation of Chk2 in an ataxia telangiectasia mutated-dependent manner.
Poly(ADP-ribose) polymerase activity prevents signaling pathways for cell cycle arrest after DNA methylating agent exposure.
Burger K, Schlackow M, Gullerova M
Nucleic acids research 2019 Apr 23;47(7):3467-3484
Nucleic acids research 2019 Apr 23;47(7):3467-3484
Melatonin Sensitizes Hepatocellular Carcinoma Cells to Chemotherapy Through Long Non-Coding RNA RAD51-AS1-Mediated Suppression of DNA Repair.
Chen CC, Chen CY, Wang SH, Yeh CT, Su SC, Ueng SH, Chuang WY, Hsueh C, Wang TH
Cancers 2018 Sep 10;10(9)
Cancers 2018 Sep 10;10(9)
TP53 Haploinsufficiency Rescues Emergency Granulopoiesis in FANCC-/- Mice.
Hu L, Huang W, Bei L, Broglie L, Eklund EA
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 15;200(6):2129-2139
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 15;200(6):2129-2139
The anticancer effects of MPT0G211, a novel HDAC6 inhibitor, combined with chemotherapeutic agents in human acute leukemia cells.
Tu HJ, Lin YJ, Chao MW, Sung TY, Wu YW, Chen YY, Lin MH, Liou JP, Pan SL, Yang CR
Clinical epigenetics 2018 Dec 29;10(1):162
Clinical epigenetics 2018 Dec 29;10(1):162
Checkpoint kinase 2 is dispensable for regulation of the p53 response but is required for G2/M arrest and cell survival in cells with p53 defects under heat stress.
Furusawa Y, Yamanouchi Y, Iizumi T, Zhao QL, Mitsuhashi Y, Morita A, Enomoto A, Tabuchi Y, Kondo T
Apoptosis : an international journal on programmed cell death 2017 Oct;22(10):1225-1234
Apoptosis : an international journal on programmed cell death 2017 Oct;22(10):1225-1234
RPA inhibition increases replication stress and suppresses tumor growth.
Glanzer JG, Liu S, Wang L, Mosel A, Peng A, Oakley GG
Cancer research 2014 Sep 15;74(18):5165-72
Cancer research 2014 Sep 15;74(18):5165-72
RAD50 phosphorylation promotes ATR downstream signaling and DNA restart following replication stress.
Gatei M, Kijas AW, Biard D, Dörk T, Lavin MF
Human molecular genetics 2014 Aug 15;23(16):4232-48
Human molecular genetics 2014 Aug 15;23(16):4232-48
Ataxia telangiectasia-mutated- and Rad3-related protein regulates the DNA damage-induced G2/M checkpoint through the Aurora A cofactor Bora protein.
Qin B, Gao B, Yu J, Yuan J, Lou Z
The Journal of biological chemistry 2013 May 31;288(22):16139-44
The Journal of biological chemistry 2013 May 31;288(22):16139-44
Inhibition of checkpoint kinase 1 abrogates G2/M checkpoint activation and promotes apoptosis under heat stress.
Furusawa Y, Iizumi T, Fujiwara Y, Zhao QL, Tabuchi Y, Nomura T, Kondo T
Apoptosis : an international journal on programmed cell death 2012 Jan;17(1):102-12
Apoptosis : an international journal on programmed cell death 2012 Jan;17(1):102-12
Hsp90 inhibitor-mediated disruption of chaperone association of ATR with hsp90 sensitizes cancer cells to DNA damage.
Ha K, Fiskus W, Rao R, Balusu R, Venkannagari S, Nalabothula NR, Bhalla KN
Molecular cancer therapeutics 2011 Jul;10(7):1194-206
Molecular cancer therapeutics 2011 Jul;10(7):1194-206
Nek1 kinase functions in DNA damage response and checkpoint control through a pathway independent of ATM and ATR.
Chen Y, Chen CF, Riley DJ, Chen PL
Cell cycle (Georgetown, Tex.) 2011 Feb 15;10(4):655-63
Cell cycle (Georgetown, Tex.) 2011 Feb 15;10(4):655-63
Nek1 kinase functions in DNA damage response and checkpoint control through a pathway independent of ATM and ATR.
Chen Y, Chen CF, Riley DJ, Chen PL
Cell cycle (Georgetown, Tex.) 2011 Feb 15;10(4):655-63
Cell cycle (Georgetown, Tex.) 2011 Feb 15;10(4):655-63
ATM- and ATR-mediated response to DNA damage induced by a novel camptothecin, ST1968.
Zuco V, Benedetti V, Zunino F
Cancer letters 2010 Jun 28;292(2):186-96
Cancer letters 2010 Jun 28;292(2):186-96
Bifunctional DNA alkylator 1,3-bis(2-chloroethyl)-1-nitrosourea activates the ATR-Chk1 pathway independently of the mismatch repair pathway.
Cui B, Johnson SP, Bullock N, Ali-Osman F, Bigner DD, Friedman HS
Molecular pharmacology 2009 Jun;75(6):1356-63
Molecular pharmacology 2009 Jun;75(6):1356-63
Interaction between PARP-1 and ATR in mouse fibroblasts is blocked by PARP inhibition.
Kedar PS, Stefanick DF, Horton JK, Wilson SH
DNA repair 2008 Nov 1;7(11):1787-98
DNA repair 2008 Nov 1;7(11):1787-98
Differential roles of ATR and ATM in p53, Chk1, and histone H2AX phosphorylation in response to hyperoxia: ATR-dependent ATM activation.
Kulkarni A, Das KC
American journal of physiology. Lung cellular and molecular physiology 2008 May;294(5):L998-L1006
American journal of physiology. Lung cellular and molecular physiology 2008 May;294(5):L998-L1006
Ataxia-telangiectasia and Rad3-related and DNA-dependent protein kinase cooperate in G2 checkpoint activation by the DNA strand-breaking nucleoside analogue 2'-C-cyano-2'-deoxy-1-beta-D-arabino-pentofuranosylcytosine.
Liu X, Matsuda A, Plunkett W
Molecular cancer therapeutics 2008 Jan;7(1):133-42
Molecular cancer therapeutics 2008 Jan;7(1):133-42
Caffeine yields aneuploidy through asymmetrical cell division caused by misalignment of chromosomes.
Katsuki Y, Nakada S, Yokoyama T, Imoto I, Inazawa J, Nagasawa M, Mizutani S
Cancer science 2008 Aug;99(8):1539-45
Cancer science 2008 Aug;99(8):1539-45
ATR-dependent checkpoint modulates XPA nuclear import in response to UV irradiation.
Wu X, Shell SM, Liu Y, Zou Y
Oncogene 2007 Feb 1;26(5):757-64
Oncogene 2007 Feb 1;26(5):757-64
NBS1 mediates ATR-dependent RPA hyperphosphorylation following replication-fork stall and collapse.
Manthey KC, Opiyo S, Glanzer JG, Dimitrova D, Elliott J, Oakley GG
Journal of cell science 2007 Dec 1;120(Pt 23):4221-9
Journal of cell science 2007 Dec 1;120(Pt 23):4221-9
DNA damage responses in progeroid syndromes arise from defective maturation of prelamin A.
Liu Y, Rusinol A, Sinensky M, Wang Y, Zou Y
Journal of cell science 2006 Nov 15;119(Pt 22):4644-9
Journal of cell science 2006 Nov 15;119(Pt 22):4644-9
Phosphorylation of nucleotide excision repair factor xeroderma pigmentosum group A by ataxia telangiectasia mutated and Rad3-related-dependent checkpoint pathway promotes cell survival in response to UV irradiation.
Wu X, Shell SM, Yang Z, Zou Y
Cancer research 2006 Mar 15;66(6):2997-3005
Cancer research 2006 Mar 15;66(6):2997-3005
Regulation of CHK2 by DNA-dependent protein kinase.
Li J, Stern DF
The Journal of biological chemistry 2005 Mar 25;280(12):12041-50
The Journal of biological chemistry 2005 Mar 25;280(12):12041-50
Hypoxia-induced phosphorylation of Chk2 in an ataxia telangiectasia mutated-dependent manner.
Gibson SL, Bindra RS, Glazer PM
Cancer research 2005 Dec 1;65(23):10734-41
Cancer research 2005 Dec 1;65(23):10734-41
Poly(ADP-ribose) polymerase activity prevents signaling pathways for cell cycle arrest after DNA methylating agent exposure.
Horton JK, Stefanick DF, Naron JM, Kedar PS, Wilson SH
The Journal of biological chemistry 2005 Apr 22;280(16):15773-85
The Journal of biological chemistry 2005 Apr 22;280(16):15773-85
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Detection of ATM (Anti-ATM 2C1, GTX70103) or ATR (Anti-ATR 2B5,GTX70109) by western blot in human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ATR antibody detects ATR protein by western blot analysis. Raji whole cell extracts and nuclear extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody (GTX670109) at a dilution of 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Raji whole cell and nuclear extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody (GTX70109) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] (GTX70109) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa, using ATR(GTX70109) antibody at 1:100 dilution.
