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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- TA319394 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-ATR antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-ATR antibody
- Reactivity
- Canine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ATR
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.28 mg/mL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Western blot using anti-ATR antibody shows detection of ATR in HeLa cell nuclear extract (lane 1). Lane 2 shows negligible staining after pre-incubation of antibody with the immunizing peptide (50 ?g peptide for 1 h at room temperature followed by centrifugation). A 4-8% gradient gel was used for separation. Goat serum was used at 5% for blocking. The arrowhead corresponds to 301 kDa ATR when compared to MW markers (Lane M). The primary antibody was used at a 1:1,400 dilution.
- Validation comment
- WB
