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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NBP2-43564 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ATR Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Untreated (-) and treated (+) HCT116 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [222]. The signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Analysis of ATR protein from HCT-116 whole cell extracts using 5 ug of ATR antibody or ATR antibody. Western blot analysis was performed using ATR antibody. EasyBlot anti-Mouse IgG was used as a secondary reagent.
