710746

antibody from Invitrogen Antibodies

Targeting: ATR FRP1, MEC1, SCKL, SCKL1

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

710746

Provider

Invitrogen Antibodies

Product name

ATR Recombinant Polyclonal Antibody (23HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Cat and Bovine.

Antibody clone number

23HCLC

Concentration

0.5 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, Hydroxy Urea (3 mM, 20h) treated HeLa cells were fixed and permeabilized for detection of ATR using ATR Recombinant Rabbit Polyclonal Antibody (Product # 710746, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of ATR protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear and nuclear localization of ATR. Panel e) shows untreated cells with cytoplasmic localization. Panel f) represents control cells with no primary antibody to assess background.