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- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [10]
- Other assay [1]
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- Product number
- PA5-77873 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-ATR (Thr1989) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: HCT116, HCT116 treated 1mM Hydroxyurea, Raji, Raji nuclear extract Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.09 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-ATR (Thr1989) in Raji cells using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with Phospho-ATR (Thr1989) polyclonal antibody (Product # PA5-77873) using a dilution of 1:500 followed by HRP-conjugated anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-ATR (Thr1989) in untreated (-) and treated (+) HCT-116 cells using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with Phospho-ATR (Thr1989) polyclonal antibody (Product # PA5-77873) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873). Untreated (–) and treated (+) HCT-116 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873). Raji whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Phospho-ATR (Thr1989) was performed by separating 30 µg of untreated (–) and treated (+) HCT116 whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873) at a dilution of 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Phospho-ATR-(Thr1989) was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873) diluted at 1:500. Red: phalloidin, a cytoskeleton marker.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Phospho-ATR (Thr1989) Polyclonal Antibody detects ATR (phospho Thr1989) protein at nucleus in human lung by immunohistochemical analysis. Sample: Paraffin-embedded human lung. Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Phospho-ATR (Thr1989) Polyclonal Antibody detects ATR (phospho Thr1989) protein at nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Phospho-ATR (Thr1989) Polyclonal Antibody detects ATR (phospho Thr1989) protein at nucleus in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Phospho-ATR (Thr1989) Polyclonal Antibody detects ATR (phospho Thr1989) protein at nucleus in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Phospho-ATR (Thr1989) Polyclonal Antibody detects ATR protein at nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Phospho-ATR (Thr1989) was performed in paraffin-embedded human ovarian cancer tissue using Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Phospho-ATR (Thr1989) was performed in paraffin-embedded human esophageal carcinoma tissue using Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Phospho-ATR (Thr1989) Polyclonal Antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ATR (phospho Thr1989) stained by Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Phospho-ATR (Thr1989) Polyclonal Antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ATR (phospho Thr1989) stained by Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Phospho-ATR (Thr1989) Polyclonal Antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. ATR (phospho Thr1989) stained by Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ATR (phospho Thr1989) was performed in HCT-116 whole cell extracts treated with Hydroxyurea for 6 hr using 5 µg of Phospho-ATR (Thr1989) Polyclonal Antibody (Product # PA5-77873). Samples were transferred to a membrane and probed with Phospho-ATR (Thr1989) Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
