PA5-85507

antibody from Invitrogen Antibodies

Targeting: ATR FRP1, MEC1, SCKL, SCKL1

Antibody data

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Validation data

Product number: PA5-85507 - Provider product page
Provider: Invitrogen Antibodies
Product name: ATR Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Keep as concentrated solution. Positive Control: 293T, A431, HeLa, HepG2, Raji, Raji NE, Raji nuclear extract. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ATR protein structure - PA5-85507 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ATR Polyclonal Antibody detects ATR protein by western blot analysis. Raji whole cell extracts and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR Polyclonal Antibody (Product # PA5-85507) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ATR Polyclonal Antibody detects ATR protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR Polyclonal Antibody (Product # PA5-85507) diluted at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ATR in Raji whole cell extracts and nuclear extracts using ATR polyclonal antibody (Product # PA5-85507) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ATR in various whole cell extracts using ATR polyclonal antibody (Product # PA5-85507) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ATR in Raji whole cell and nuclear extracts using ATR polyclonal antibody (Product # PA5-85507) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.