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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-85507 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Keep as concentrated solution.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATR in various whole cell extracts using ATR polyclonal antibody (Product # PA5-85507) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATR in various whole cell extracts using ATR polyclonal antibody (Product # PA5-85507) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATR in Raji whole cell extracts and nuclear extracts using ATR polyclonal antibody (Product # PA5-85507) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATR Polyclonal Antibody detects ATR protein by western blot analysis. Raji whole cell extracts and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR Polyclonal Antibody (Product # PA5-85507) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATR in Raji whole cell and nuclear extracts using ATR polyclonal antibody (Product # PA5-85507) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATR Polyclonal Antibody detects ATR protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR Polyclonal Antibody (Product # PA5-85507) diluted at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATR Polyclonal Antibody detects ATR protein at nucleolus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATR protein stained by ATR Polyclonal Antibody (Product # PA5-85507) diluted at 1:5,000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATR Polyclonal Antibody detects ATR protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. ATR Polyclonal Antibody (Product # PA5-85507) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATR Polyclonal Antibody detects ATR protein at nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. ATR Polyclonal Antibody (Product # PA5-85507) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of ATR in HCT-116 whole cell extracts with ATR polyclonal antibody (Product # PA5-85507) using 5 µg of sample, followed by anti-Rabbit IgG secondary antibody.
