Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-450 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- PA1-450 detects ATR (Ataxia Telangiectasia Mutated (ATM) and Rad3-related protein) from human cells. PA1-450 has been successfully used in Western blot immunofluoresence and immunoprecipitation procedures. By Western blot, this antibody detects an ~305 kDa protein representing ATR in lysate from UV irradiated K562 cells. PA1-450 antigen is a fusion protein corresponding to amino acid residues 400-460 from human ATR.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 4 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Loss of MAP3K7 Sensitizes Prostate Cancer Cells to CDK1/2 Inhibition and DNA Damage by Disrupting Homologous Recombination.
The Ataxia telangiectasia-mutated and Rad3-related protein kinase regulates cellular hydrogen sulfide concentrations.
Atrazine Triggers DNA Damage Response and Induces DNA Double-Strand Breaks in MCF-10A Cells.
Role for casein kinase 1 in the phosphorylation of Claspin on critical residues necessary for the activation of Chk1.
ATR preferentially interacts with common fragile site FRA3B and the binding requires its kinase activity in response to aphidicolin treatment.
ATR-dependent phosphorylation of FANCA on serine 1449 after DNA damage is important for FA pathway function.
DNA-damage response, survival and differentiation in vitro of a human neural stem cell line in relation to ATM expression.
p300/CREB-binding protein interacts with ATR and is required for the DNA replication checkpoint.
BRCA1 activates a G2-M cell cycle checkpoint following 6-thioguanine-induced DNA mismatch damage.
The DNA damage machinery and homologous recombination pathway act consecutively to protect human telomeres.
Determination of the catalytic activities of mTOR and other members of the phosphoinositide-3-kinase-related kinase family.
Interaction between human MCM7 and Rad17 proteins is required for replication checkpoint signaling.
Mismatch repair-mediated G2/M arrest by 6-thioguanine involves the ATR-Chk1 pathway.
The ATR-p53 pathway is suppressed in noncycling normal and malignant lymphocytes.
Quaternary structure of ATR and effects of ATRIP and replication protein A on its DNA binding and kinase activities.
DNA replication defects, spontaneous DNA damage, and ATM-dependent checkpoint activation in replication protein A-deficient cells.
ATR functions as a gene dosage-dependent tumor suppressor on a mismatch repair-deficient background.
DNA damage-induced cell-cycle phase regulation of p53 and p21waf1 in normal and ATM-defective cells.
ATP activates ataxia-telangiectasia mutated (ATM) in vitro. Importance of autophosphorylation.
ATP activates ataxia-telangiectasia mutated (ATM) in vitro. Importance of autophosphorylation.
An ATR- and Chk1-dependent S checkpoint inhibits replicon initiation following UVC-induced DNA damage.
An ATR- and Chk1-dependent S checkpoint inhibits replicon initiation following UVC-induced DNA damage.
Molecular association between ATR and two components of the nucleosome remodeling and deacetylating complex, HDAC2 and CHD4.
ATR is a caffeine-sensitive, DNA-activated protein kinase with a substrate specificity distinct from DNA-PK.
Washino S, Rider LC, Romero L, Jillson LK, Affandi T, Ohm AM, Lam ET, Reyland ME, Costello JC, Cramer SD
Molecular cancer research : MCR 2019 Oct;17(10):1985-1998
Molecular cancer research : MCR 2019 Oct;17(10):1985-1998
The Ataxia telangiectasia-mutated and Rad3-related protein kinase regulates cellular hydrogen sulfide concentrations.
Chen J, Shen X, Pardue S, Meram AT, Rajendran S, Ghali GE, Kevil CG, Shackelford RE
DNA repair 2019 Jan;73:55-63
DNA repair 2019 Jan;73:55-63
Atrazine Triggers DNA Damage Response and Induces DNA Double-Strand Breaks in MCF-10A Cells.
Huang P, Yang J, Ning J, Wang M, Song Q
International journal of molecular sciences 2015 Jun 24;16(7):14353-68
International journal of molecular sciences 2015 Jun 24;16(7):14353-68
Role for casein kinase 1 in the phosphorylation of Claspin on critical residues necessary for the activation of Chk1.
Meng Z, Capalbo L, Glover DM, Dunphy WG
Molecular biology of the cell 2011 Aug 15;22(16):2834-47
Molecular biology of the cell 2011 Aug 15;22(16):2834-47
ATR preferentially interacts with common fragile site FRA3B and the binding requires its kinase activity in response to aphidicolin treatment.
Wan C, Kulkarni A, Wang YH
Mutation research 2010 Apr 1;686(1-2):39-46
Mutation research 2010 Apr 1;686(1-2):39-46
ATR-dependent phosphorylation of FANCA on serine 1449 after DNA damage is important for FA pathway function.
Collins NB, Wilson JB, Bush T, Thomashevski A, Roberts KJ, Jones NJ, Kupfer GM
Blood 2009 Mar 5;113(10):2181-90
Blood 2009 Mar 5;113(10):2181-90
DNA-damage response, survival and differentiation in vitro of a human neural stem cell line in relation to ATM expression.
Carlessi L, De Filippis L, Lecis D, Vescovi A, Delia D
Cell death and differentiation 2009 Jun;16(6):795-806
Cell death and differentiation 2009 Jun;16(6):795-806
p300/CREB-binding protein interacts with ATR and is required for the DNA replication checkpoint.
Stauffer D, Chang B, Huang J, Dunn A, Thayer M
The Journal of biological chemistry 2007 Mar 30;282(13):9678-87
The Journal of biological chemistry 2007 Mar 30;282(13):9678-87
BRCA1 activates a G2-M cell cycle checkpoint following 6-thioguanine-induced DNA mismatch damage.
Yamane K, Schupp JE, Kinsella TJ
Cancer research 2007 Jul 1;67(13):6286-92
Cancer research 2007 Jul 1;67(13):6286-92
The DNA damage machinery and homologous recombination pathway act consecutively to protect human telomeres.
Verdun RE, Karlseder J
Cell 2006 Nov 17;127(4):709-20
Cell 2006 Nov 17;127(4):709-20
Determination of the catalytic activities of mTOR and other members of the phosphoinositide-3-kinase-related kinase family.
Chiang GG, Abraham RT
Methods in molecular biology (Clifton, N.J.) 2004;281:125-41
Methods in molecular biology (Clifton, N.J.) 2004;281:125-41
Interaction between human MCM7 and Rad17 proteins is required for replication checkpoint signaling.
Tsao CC, Geisen C, Abraham RT
The EMBO journal 2004 Nov 24;23(23):4660-9
The EMBO journal 2004 Nov 24;23(23):4660-9
Mismatch repair-mediated G2/M arrest by 6-thioguanine involves the ATR-Chk1 pathway.
Yamane K, Taylor K, Kinsella TJ
Biochemical and biophysical research communications 2004 May 21;318(1):297-302
Biochemical and biophysical research communications 2004 May 21;318(1):297-302
The ATR-p53 pathway is suppressed in noncycling normal and malignant lymphocytes.
Jones GG, Reaper PM, Pettitt AR, Sherrington PD
Oncogene 2004 Mar 11;23(10):1911-21
Oncogene 2004 Mar 11;23(10):1911-21
Quaternary structure of ATR and effects of ATRIP and replication protein A on its DNA binding and kinase activities.
Unsal-Kaçmaz K, Sancar A
Molecular and cellular biology 2004 Feb;24(3):1292-300
Molecular and cellular biology 2004 Feb;24(3):1292-300
DNA replication defects, spontaneous DNA damage, and ATM-dependent checkpoint activation in replication protein A-deficient cells.
Dodson GE, Shi Y, Tibbetts RS
The Journal of biological chemistry 2004 Aug 6;279(32):34010-4
The Journal of biological chemistry 2004 Aug 6;279(32):34010-4
ATR functions as a gene dosage-dependent tumor suppressor on a mismatch repair-deficient background.
Fang Y, Tsao CC, Goodman BK, Furumai R, Tirado CA, Abraham RT, Wang XF
The EMBO journal 2004 Aug 4;23(15):3164-74
The EMBO journal 2004 Aug 4;23(15):3164-74
DNA damage-induced cell-cycle phase regulation of p53 and p21waf1 in normal and ATM-defective cells.
Delia D, Fontanella E, Ferrario C, Chessa L, Mizutani S
Oncogene 2003 Oct 30;22(49):7866-9
Oncogene 2003 Oct 30;22(49):7866-9
ATP activates ataxia-telangiectasia mutated (ATM) in vitro. Importance of autophosphorylation.
Kozlov S, Gueven N, Keating K, Ramsay J, Lavin MF
The Journal of biological chemistry 2003 Mar 14;278(11):9309-17
The Journal of biological chemistry 2003 Mar 14;278(11):9309-17
ATP activates ataxia-telangiectasia mutated (ATM) in vitro. Importance of autophosphorylation.
Kozlov S, Gueven N, Keating K, Ramsay J, Lavin MF
The Journal of biological chemistry 2003 Mar 14;278(11):9309-17
The Journal of biological chemistry 2003 Mar 14;278(11):9309-17
An ATR- and Chk1-dependent S checkpoint inhibits replicon initiation following UVC-induced DNA damage.
Heffernan TP, Simpson DA, Frank AR, Heinloth AN, Paules RS, Cordeiro-Stone M, Kaufmann WK
Molecular and cellular biology 2002 Dec;22(24):8552-61
Molecular and cellular biology 2002 Dec;22(24):8552-61
An ATR- and Chk1-dependent S checkpoint inhibits replicon initiation following UVC-induced DNA damage.
Heffernan TP, Simpson DA, Frank AR, Heinloth AN, Paules RS, Cordeiro-Stone M, Kaufmann WK
Molecular and cellular biology 2002 Dec;22(24):8552-61
Molecular and cellular biology 2002 Dec;22(24):8552-61
Molecular association between ATR and two components of the nucleosome remodeling and deacetylating complex, HDAC2 and CHD4.
Schmidt DR, Schreiber SL
Biochemistry 1999 Nov 2;38(44):14711-7
Biochemistry 1999 Nov 2;38(44):14711-7
ATR is a caffeine-sensitive, DNA-activated protein kinase with a substrate specificity distinct from DNA-PK.
Hall-Jackson CA, Cross DA, Morrice N, Smythe C
Oncogene 1999 Nov 18;18(48):6707-13
Oncogene 1999 Nov 18;18(48):6707-13
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot on ATR in K562 whole cell lysate using Product # PA1-450.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATR was performed with 10 µg of HeLa cells transfected with Transfection Reagent alone (Lane 1), 100nM Non-Targeting control siRNA (Lane 2), or 100nM siRNA against ATR (Lane 3). Proteins were resolved using a NuPAGE® Novex 4-12% Bis-Tris Gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and a protein size ladder. Proteins were wet transferred to a Pierce Nitrocellulose Membrane (Product # 88025) OR Pierce PVDF Membrane (Product # 88518) and blocked with Pierce Starting Block T20 (PBS) Blocking Buffer (Product # 37539) for 1 hour at room temperature. ATR was detected at ~ 305 kDa using ATR Rabbit polyclonal antibody (Product # PA1-450) diluted in Pierce Starting Block T20 (PBS) Blocking Buffer 4°C overnight on a rocking platform. Pierce Goat Anti-Rabbit (Product # 31461) HRP-Conjugated Antibodies at a 1:2500 dilution were used and chemiluminescent detection was performed using Pierce Supersignal West Dura Maximum Sensitivity Substrate (Product # 37071). Relative density of the bands normalized to GAPDH (36 kDa). ATR Antibody (Product # PA1-450) confirms silencing of ATR expression.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Atrazine-induced expression of ATR, ATRIP and phosphorylation of Chk1 (Ser317) in MCF-10A cells. ( A ) Dose-response of atrazine-induced ATR, ATRIP and p-Chk1. MCF-10A cells (3 x 10 5 ) were incubated with the indicated concentrations of atrazine for 6 h and analyzed by Western blot; ( B ) time course of atrazine-induced ATR, ATRIP and p-Chk1in MCF-10A cells. MCF-10A cells (3 x 10 5 ) were treated with 0.1 mug/mL of atrazine for the indicated times and analyzed by Western blot. For each blot, the resulting fold-change of atrazine-mediated ATR, ATRIP and p-Chk1 expression over DMSO untreated controls is provided in the corresponding histogram ( n = 3) with the signal normalized to actin as a loading control. * p < 0.05, ** p < 0.01 vs. controls.
