NB100-308

antibody from Novus Biologicals

Targeting: ATR FRP1, MEC1, SCKL, SCKL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Functional assay (Data presented on provider website)

Antibody Data

Product number

NB100-308

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-308, RRID:AB_10000618

Product name

Mouse Monoclonal ATR Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

ZFP161 regulates replication fork stability and maintenance of genomic stability by recruiting the ATR/ATRIP complex.

Kim W, Zhao F, Wu R, Qin S, Nowsheen S, Huang J, Zhou Q, Chen Y, Deng M, Guo G, Luo K, Lou Z, Yuan J
Nature communications 2019 Nov 22;10(1):5304

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Dysregulation of IRP1-mediated iron metabolism causes gamma ray-specific radioresistance in leukemia cells.

Haro KJ, Sheth A, Scheinberg DA
PloS one 2012;7(11):e48841

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Constitutive turnover of phosphorylation at Thr-412 of human p57/coronin-1 regulates the interaction with actin.

Oku T, Nakano M, Kaneko Y, Ando Y, Kenmotsu H, Itoh S, Tsuiji M, Seyama Y, Toyoshima S, Tsuji T
The Journal of biological chemistry 2012 Dec 14;287(51):42910-20

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Hsp90 inhibitor-mediated disruption of chaperone association of ATR with hsp90 sensitizes cancer cells to DNA damage.

Ha K, Fiskus W, Rao R, Balusu R, Venkannagari S, Nalabothula NR, Bhalla KN
Molecular cancer therapeutics 2011 Jul;10(7):1194-206

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Nek1 kinase functions in DNA damage response and checkpoint control through a pathway independent of ATM and ATR.

Chen Y, Chen CF, Riley DJ, Chen PL
Cell cycle (Georgetown, Tex.) 2011 Feb 15;10(4):655-63

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ATM- and ATR-mediated response to DNA damage induced by a novel camptothecin, ST1968.

Zuco V, Benedetti V, Zunino F
Cancer letters 2010 Jun 28;292(2):186-96

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Bifunctional DNA alkylator 1,3-bis(2-chloroethyl)-1-nitrosourea activates the ATR-Chk1 pathway independently of the mismatch repair pathway.

Cui B, Johnson SP, Bullock N, Ali-Osman F, Bigner DD, Friedman HS
Molecular pharmacology 2009 Jun;75(6):1356-63

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Differential roles of ATR and ATM in p53, Chk1, and histone H2AX phosphorylation in response to hyperoxia: ATR-dependent ATM activation.

Kulkarni A, Das KC
American journal of physiology. Lung cellular and molecular physiology 2008 May;294(5):L998-L1006

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Ataxia-telangiectasia and Rad3-related and DNA-dependent protein kinase cooperate in G2 checkpoint activation by the DNA strand-breaking nucleoside analogue 2'-C-cyano-2'-deoxy-1-beta-D-arabino-pentofuranosylcytosine.

Liu X, Matsuda A, Plunkett W
Molecular cancer therapeutics 2008 Jan;7(1):133-42

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Caffeine yields aneuploidy through asymmetrical cell division caused by misalignment of chromosomes.

Katsuki Y, Nakada S, Yokoyama T, Imoto I, Inazawa J, Nagasawa M, Mizutani S
Cancer science 2008 Aug;99(8):1539-45

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ATR-dependent checkpoint modulates XPA nuclear import in response to UV irradiation.

Wu X, Shell SM, Liu Y, Zou Y
Oncogene 2007 Feb 1;26(5):757-64

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NBS1 mediates ATR-dependent RPA hyperphosphorylation following replication-fork stall and collapse.

Manthey KC, Opiyo S, Glanzer JG, Dimitrova D, Elliott J, Oakley GG
Journal of cell science 2007 Dec 1;120(Pt 23):4221-9

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Silibinin activates p53-caspase 2 pathway and causes caspase-mediated cleavage of Cip1/p21 in apoptosis induction in bladder transitional-cell papilloma RT4 cells: evidence for a regulatory loop between p53 and caspase 2.

Tyagi A, Singh RP, Agarwal C, Agarwal R
Carcinogenesis 2006 Nov;27(11):2269-80

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DNA damage responses in progeroid syndromes arise from defective maturation of prelamin A.

Liu Y, Rusinol A, Sinensky M, Wang Y, Zou Y
Journal of cell science 2006 Nov 15;119(Pt 22):4644-9

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Phosphorylation of nucleotide excision repair factor xeroderma pigmentosum group A by ataxia telangiectasia mutated and Rad3-related-dependent checkpoint pathway promotes cell survival in response to UV irradiation.

Wu X, Shell SM, Yang Z, Zou Y
Cancer research 2006 Mar 15;66(6):2997-3005

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Regulation of CHK2 by DNA-dependent protein kinase.

Li J, Stern DF
The Journal of biological chemistry 2005 Mar 25;280(12):12041-50

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Hypoxia-induced phosphorylation of Chk2 in an ataxia telangiectasia mutated-dependent manner.

Gibson SL, Bindra RS, Glazer PM
Cancer research 2005 Dec 1;65(23):10734-41

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Poly(ADP-ribose) polymerase activity prevents signaling pathways for cell cycle arrest after DNA methylating agent exposure.

Horton JK, Stefanick DF, Naron JM, Kedar PS, Wilson SH
The Journal of biological chemistry 2005 Apr 22;280(16):15773-85

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATR Antibody (2B5) [NB100-308] - Raji whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.