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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09371PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09371PU-N, RRID:AB_2035154
- Product name
- anti ATR / FRP1
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to an internal region of human ATR protein
- Reactivity
- Human, Mouse, Rat, Canine, Simian, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.28 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using anti-ATR antibody shows detection of ATR in HeLa cell nuclear extract (lane 1). Lane 2 shows negligible staining after pre-incubation of antibody with the immunizing peptide (50 µg peptide for 1 h at room temperature followed by centrifugation). A 4-8% gradient gel was used for separation. Goat serum was used at 5% for blocking. The arrowhead corresponds to 301 kDa ATR when compared to MW markers (Lane M). The primary antibody was used at a 1:1,400 dilution.
