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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Other assay [2]
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- Product number
- PA5-53761 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHAD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LRWLYLSENA LSSLQPGALD DVENLAKFHV DRNQLSSYPS AALSKLRVVE ELKLSHNPLK SIPDNAFQSF GRYLETLWLD NTNLEKFSDG AFLGVTT Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 94%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references LPS-Induced Inflammation Prior to Injury Exacerbates the Development of Post-Traumatic Osteoarthritis in Mice.
Global Gene Expression Analysis Identifies Age-Related Differences in Knee Joint Transcriptome during the Development of Post-Traumatic Osteoarthritis in Mice.
Mendez ME, Sebastian A, Murugesh DK, Hum NR, McCool JL, Hsia AW, Christiansen BA, Loots GG
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2020 Nov;35(11):2229-2241
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2020 Nov;35(11):2229-2241
Global Gene Expression Analysis Identifies Age-Related Differences in Knee Joint Transcriptome during the Development of Post-Traumatic Osteoarthritis in Mice.
Sebastian A, Murugesh DK, Mendez ME, Hum NR, Rios-Arce ND, McCool JL, Christiansen BA, Loots GG
International journal of molecular sciences 2020 Jan 6;21(1)
International journal of molecular sciences 2020 Jan 6;21(1)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHAD in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a CHAD Polyclonal Antibody (Product # PA5-53761).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHAD in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a CHAD Polyclonal Antibody (Product # PA5-53761).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CHAD in human cervix, uterine and tonsil tissues using CHAD Polyclonal Antibody (Product # PA5-53761). Corresponding CHAD RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CHAD in human cervix, uterine using CHAD Polyclonal Antibody (Product # PA5-53761) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CHAD in human tonsil using CHAD Polyclonal Antibody (Product # PA5-53761) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Chad ( A ) and Plod2 ( B ) gene expression at six-week post injury in 10-week-old and 62-week-old mice. Chad ( C ) and Plod2 ( D ) protein expression in 10-week-old and 62-week-old mice Green: protein staining. Blue: DAPI staining marking the nucleus. (40x magnification).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 5 Fig Gene expression changes caused by LPS and injury 24 hours post-injury. ( A ) Inflammatory/immune system genes upregulated in response to LPS treatment and injury in males. ( B ) Immune/inflammatory response genes upregulated in response to LPS treatment and injury in females. ( C ) Bone and cartilage formation genes downregulated in response to LPS treatment and injury in males. ( D ) Injured C57Bl/6J VEH male CYTL1 immunohistochemistry expression. ( E ) Injured C57Bl/6J LPS male CYTL1 immunohistochemistry expression is lower than VEH. ( F ) Injured C57Bl/6J VEH CHAD immunohistochemistry expression. ( G ) Injured C57Bl/6J LPS CHAD immunohistochemistry expression was lower than VEH.
