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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX109571 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109571, RRID:AB_1949620
- Product name
- AKR1B10 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AKR1B10 antibody detects AKR1B10 protein by western blot analysis.A. 50 ?g mouse colon lysate/extract10% SDS-PAGEAKR1B10 antibody (GTX109571) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate)A: MOLT4 (GTX27912)B: Raji (GTX27908)10% SDS PAGEGTX109571 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AKR1B10 antibody detects AKR1B10 protein by western blot analysis.A. 50 ?g rat colon lysate/extract10% SDS-PAGEAKR1B10 antibody (GTX109571) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AKR1B10 antibody detects AKR1B10 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with AKR1B10 antibody (GTX109571) diluted by 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKR1B10(GTX109571) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using AKR1B10(GTX109571) antibody at 1:500 dilution.
