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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00022978-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00022978-M02, RRID:AB_922125
- Product name
- Mouse Monoclonal NT5C2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. NT5C2 - 5'
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references SAMHD1 is a biomarker for cytarabine response and a therapeutic target in acute myeloid leukemia.
Cytosolic 5'-nucleotidase II interacts with the leucin rich repeat of NLR family member Ipaf.
Determination of the enzymatic activity of cytosolic 5'-nucleotidase cN-II in cancer cells: development of a simple analytical method and related cell line models.
Cell proliferation and drug sensitivity of human glioblastoma cells are altered by the stable modulation of cytosolic 5'-nucleotidase II.
Suppression of 5'-nucleotidase enzymes promotes AMP-activated protein kinase (AMPK) phosphorylation and metabolism in human and mouse skeletal muscle.
Schneider C, Oellerich T, Baldauf HM, Schwarz SM, Thomas D, Flick R, Bohnenberger H, Kaderali L, Stegmann L, Cremer A, Martin M, Lohmeyer J, Michaelis M, Hornung V, Schliemann C, Berdel WE, Hartmann W, Wardelmann E, Comoglio F, Hansmann ML, Yakunin AF, Geisslinger G, Ströbel P, Ferreirós N, Serve H, Keppler OT, Cinatl J Jr
Nature medicine 2017 Feb;23(2):250-255
Nature medicine 2017 Feb;23(2):250-255
Cytosolic 5'-nucleotidase II interacts with the leucin rich repeat of NLR family member Ipaf.
Cividini F, Tozzi MG, Galli A, Pesi R, Camici M, Dumontet C, Jordheim LP, Allegrini S
PloS one 2015;10(3):e0121525
PloS one 2015;10(3):e0121525
Determination of the enzymatic activity of cytosolic 5'-nucleotidase cN-II in cancer cells: development of a simple analytical method and related cell line models.
Jordheim LP, Puy JY, Cros-Perrial E, Peyrottes S, Lefebvre I, Périgaud C, Dumontet C
Analytical and bioanalytical chemistry 2015 Jul;407(19):5747-58
Analytical and bioanalytical chemistry 2015 Jul;407(19):5747-58
Cell proliferation and drug sensitivity of human glioblastoma cells are altered by the stable modulation of cytosolic 5'-nucleotidase II.
Cividini F, Cros-Perrial E, Pesi R, Machon C, Allegrini S, Camici M, Dumontet C, Jordheim LP, Tozzi MG
The international journal of biochemistry & cell biology 2015 Aug;65:222-9
The international journal of biochemistry & cell biology 2015 Aug;65:222-9
Suppression of 5'-nucleotidase enzymes promotes AMP-activated protein kinase (AMPK) phosphorylation and metabolism in human and mouse skeletal muscle.
Kulkarni SS, Karlsson HK, Szekeres F, Chibalin AV, Krook A, Zierath JR
The Journal of biological chemistry 2011 Oct 7;286(40):34567-74
The Journal of biological chemistry 2011 Oct 7;286(40):34567-74
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NT5C2 Antibody (3C1) [H00022978-M02] - Analysis of NT5C2 expression in transfected 293T cell line by NT5C2 monoclonal antibody (M02), clone 3C1.Lane 1: NT5C2 transfected lysate(64.97 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: NT5C2 Antibody (3C1) [H00022978-M02] - Analysis of monoclonal antibody to NT5C2 on HeLa cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NT5C2 Antibody (3C1) [H00022978-M02] - Analysis of monoclonal antibody to NT5C2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
