TA324241

antibody from OriGene

Targeting: SPATA20 FLJ21347, SSP411, Tisp78

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324241

Provider

OriGene

Product name

Anti-SPATA20 rabbit polyclonal antibody

Antibody type

Polyclonal

Description

Anti-SPATA20 rabbit polyclonal antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

SPATA20

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

2.8mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 88kDa. Positive control: A549 and lncap cell, human seminoma tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: A549 cell lysate Lane2: Lncap cell lysate Lane3: Human seminoma tissue lysate Lysates: 60 ug per lane Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 5 minutes)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Nucleus. Positive control: Human esophagus cancer tissue . Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using SPATA20 antibody at dilution 1/70, on the right is treated with the synthetic peptide. (Original magnification:x200)

Validation comment

IHC