Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-52220 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR3K Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PGCGNGLIVE EGQRCHRFAC NTCPYVHNIT RKVTNRKYPK LKEVDDVLGG AAAWENVDST AESCPKCEHP RAYFMQLQTR SADEPMTTFY KCCNAQCGHR WRD Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 99%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.10 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLR3K in control (vector only transfected HEK293T lysate) and POLR3K over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a POLR3K Polyclonal Antibody (Product # PA5-52220).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of POLR3K in human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a POLR3K Polyclonal Antibody (Product # PA5-52220).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of POLR3K in human testis using a POLR3K Polyclonal Antibody (Product # PA5-52220) shows strong nuclear positivity in cells of seminiferous ducts.
