11084-1-AP

antibody from Invitrogen Antibodies

Targeting: POLR3K C11, RPC11

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

11084-1-AP

Provider

Invitrogen Antibodies

Product name

POLR3K Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MLLFCPGCG NGLIVEEGQR CHRFACNTCP YVHNITRKVT NRKYPKLKEV DDVLGGAAAW ENVDSTAESC PKCEHPRAYF MQLQTRSADE PMTTFYKCCN AQCGHRWRD (1-108 aa encoded by BC011932 )

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.24 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 11084-1-AP ( POLR3K Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed HepG2 cells using 11084-1-AP (POLR3K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using POLR3K antibody 11084-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 11084-1-AP (POLR3K antibody) at dilution of 1:100 (under 10x lens).