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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-42524 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC9A9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: INYQEQASSP CSPPARLGLD QKASPQTPGK ENIYEGDLGL GGYELKLEQT Sequence homology: Cow: 93%; Dog: 86%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 93%; Rabbit: 93%; Rat: 100%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The sodium proton exchanger NHE9 regulates phagosome maturation and bactericidal activity in macrophages.
Shamroukh HS, Lone N, Akhtar M, Altayib A, Sutliff S, Kassem Z, Tripathy SK, Kondapalli KC
The Journal of biological chemistry 2022 Aug;298(8):102150
The Journal of biological chemistry 2022 Aug;298(8):102150
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 NHE9 expression levels have no significant effect on pathogen ingestion . A , quantitative PCR analysis of stable, ectopic NHE9 expression in RAW 264.7 cells. Error bars represent standard deviation (SD), * p < 0.05. B , NHE9 expression in RAW 264.7 cells determined by immunofluorescence microscopy. Left panel : Control cells showing staining of native NHE9 protein; Right panel : Cells stably expressing NHE9 (NHE9+) Scale bar ( white line ) is 10 mum. Phagocytic ingestion by RAW 264.7 cells ( C and D ) and mouse bone marrow-derived macrophages, i.e ., BMDMs ( E and F ) after 30- or 60-min incubation with E. coli and S. aureus bacteria as indicated. Data are expressed as colony forming units (CFUs) per cell. Graphs represent mean from three biological replicates. Error bars represent standard error of the mean (SEM). NS, not statistically significant. Statistical analysis was done using Student's t -test.
