PA5-35936

antibody from Invitrogen Antibodies

Targeting: NFIA KIAA1439, NFI-L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35936

Provider

Invitrogen Antibodies

Product name

NFIA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa nuclear.

Concentration

0.4 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NFIA using 30 µg HeLa nuclear lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a NFIA polyclonal antibody (Product # PA5-35936) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NFIA Polyclonal Antibody (Product # PA5-35936). HeLa whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NFIA Polyclonal Antibody (Product # PA5-35936) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NFIA showing staining in the nucleus of A431 cells. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a NFIA polyclonal antibody (Product # PA5-35936) diluted at 1:1000. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Frozen) analysis of NFIA was performed in frozen sectioned adult mouse retina tissue using NFIA Polyclonal Antibody (Product # PA5-35936) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NFIA Polyclonal Antibody detects NFIA protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. NFIA Polyclonal Antibody (Product # PA5-35936) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NFIA Polyclonal Antibody detects NFIA protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. NFIA Polyclonal Antibody (Product # PA5-35936) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NFIA Polyclonal Antibody detects NFIA protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. NFIA Polyclonal Antibody (Product # PA5-35936) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.