Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00004774-M02-100ug - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal NFIA Antibody
- Antibody type
- Monoclonal
- Description
- Ascites. NFIA - nuclear factor I/A (1E11)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NFIA Antibody (1E11) [H00004774-M02] - Analysis of NFIA expression in transfected 293T cell line by NFIA monoclonal antibody (M02), clone 1E11.Lane 1: NFIA transfected lysate (Predicted MW: 54.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: NFIA Antibody (1E11) [H00004774-M02] - Detection limit for recombinant GST tagged NFIA is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: NFIA Antibody (1E11) [H00004774-M02] - Analysis of monoclonal antibody to NFIA on HeLa cell . Antibody concentration 10 ug/ml.