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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [10]
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- Product number
- PA5-52622 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFIA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VKSPQCSNPG LCVQPHHIGV SVKELDLYLA YFVHAADSSQ SESPSQPSDA DIKDQPENGH LGFQDSFVTS GVFSVTELVR VSQTPIAAGT GPNFSLSDLE SSSYYSMSPG A Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 100%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.10 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFIA in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a NFIA Polyclonal Antibody (Product # PA5-52622).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFIA in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a NFIA Polyclonal Antibody (Product # PA5-52622).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NFIA in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a NFIA Polyclonal Antibody (Product # PA5-52622).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NFIA in human thyroid gland using NFIA Polyclonal Antibody (Product # PA5-52622) shows strong nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NFIA in human lymph node using NFIA Polyclonal Antibody (Product # PA5-52622).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NFIA in human colon using NFIA Polyclonal Antibody (Product # PA5-52622).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NFIA in human testis using NFIA Polyclonal Antibody (Product # PA5-52622).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NFIA in human cerebral cortex using NFIA Polyclonal Antibody (Product # PA5-52622).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NFIA in human thyroid gland using a NFIA Polyclonal Antibody (Product # PA5-52622) shows nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NFIA in human cerebral cortex, colon, lymph node and testis using NFIA Polyclonal Antibody (Product # PA5-52622) (A) shows similar protein distribution across tissues to an independent NFIA Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NFIA in human prostate using NFIA Polyclonal Antibody (Product # PA5-52622) shows strong nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NFIA in human fallopian tube using NFIA Polyclonal Antibody (Product # PA5-52622) shows moderate nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NFIA in human tonsil using NFIA Polyclonal Antibody (Product # PA5-52622) shows moderate nuclear positivity in lymphoid cells.
