LS-C781879

antibody from LSBio

Targeting: NFIA KIAA1439, NFI-L

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C781879

Provider

LSBio

Product name

NFIA / Nuclear Factor 1 Antibody LS-C781879

Antibody type

Polyclonal

Description

Antigen Affinity purification

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

After reconstitution, store at 4°C for up to 1 month. Long-term: aliquot and store at -20°C. Avoid freeze-thaws cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot testing of human 1) Jurkat and 2) COLO320 lysate with NFIA antibody at 0.5ug/ml. Predicted molecular weight ~56 kDa (unmodified), 60-70 kDa (phosphorylated).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot testing of mouse 1) HEPA1-6 and 2) SP2/0 lysate with NFIA antibody at 0.5ug/ml. Predicted molecular weight ~56 kDa (unmodified), 60-70 kDa (phosphorylated).

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC testing of FFPE rat liver with NFIA antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC testing of FFPE mouse liver with NFIA antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC testing of FFPE rat liver with NFIA antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC testing of FFPE human breast cancer tissue with NFIA antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC testing of FFPE human breast cancer tissue with NFIA antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC testing of FFPE mouse liver with NFIA antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC testing of FFPE rat liver with NFIA antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.